Eberhardt Jason M, Neal Karter, Shackelford Tom, Lappin Michael R
Department of Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, 300 West Drake Road, Fort Collins, CO 80523, USA.
J Feline Med Surg. 2006 Jun;8(3):164-8. doi: 10.1016/j.jfms.2005.12.002. Epub 2006 Jan 27.
The objective of this study was to use polymerase chain reaction (PCR) assays to determine the prevalence of Ehrlichia species, Anaplasma phagocytophilum, Mycoplasma haemofelis, 'Candidatus Mycoplasma haemominutum' and Bartonella species from feral and relinquished cats in Phoenix and Nogales, Arizona. DNA from one or more of the organisms was amplified from 31 of 112 blood samples (27.7%). DNA consistent with Bartonella clarridgeiae 15 (13.4%), Bartonella henselae 14 (12.5%), 'Candidatus M haemominutum' 9 (8.0%), and M haemofelis 5 (4.5%) were detected. DNA of Ehrlichia species, Neorickettsia risticii, or A phagocytophilum was not amplified. Failure to amplify DNA of A phagocytophilum may relate to the absence of appropriate tick vectors. Failure to amplify Ehrlichia species DNA suggests that cats were not exposed, exposed but not infected, or infected but the DNA was not detected by the PCR assay used in this study. The Bartonella species and hemoplasma results suggest flea control should be maintained.
本研究的目的是使用聚合酶链反应(PCR)检测法来确定亚利桑那州凤凰城和诺加利斯的野生及被弃养猫中埃立克体属、嗜吞噬细胞无形体、溶血支原体、“嗜血红支原体”和巴尔通体属的流行情况。在112份血液样本中的31份(27.7%)检测到了一种或多种上述生物体的DNA。检测到与克莱氏巴尔通体15株(13.4%)、汉赛巴尔通体14株(12.5%)、“嗜血红支原体”9株(8.0%)和溶血支原体5株(4.5%)相符的DNA。未扩增出埃立克体属、里氏新立克次体或嗜吞噬细胞无形体的DNA。未能扩增出嗜吞噬细胞无形体的DNA可能与缺乏合适的蜱传播媒介有关。未能扩增出埃立克体属的DNA表明猫未接触过该病原体、接触过但未感染,或者感染了但本研究使用的PCR检测法未检测到其DNA。巴尔通体属和血支原体的检测结果表明应持续进行跳蚤控制。