澳大利亚东部猫和跳蚤血液中巴通体属、猫立克次体、血巴尔通体属及埃立克体属的流行情况。

Prevalence of Bartonella species, Rickettsia felis, haemoplasmas and the Ehrlichia group in the blood of cats and fleas in eastern Australia.

作者信息

Barrs V R, Beatty J A, Wilson B J, Evans N, Gowan R, Baral R M, Lingard A E, Perkovic G, Hawley J R, Lappin M R

机构信息

Valentine Charlton Cat Centre, Faculty of Veterinary Science, The University of Sydney, New South Wales 2006, Australia.

出版信息

Aust Vet J. 2010 May;88(5):160-5. doi: 10.1111/j.1751-0813.2010.00569.x.

DOI:10.1111/j.1751-0813.2010.00569.x

PMID:20529020

Abstract

OBJECTIVES

To define the prevalence of Bartonella spp., Rickettsia felis, Mycoplasma haemofelis, 'Candidatus Mycoplasma haemominutum' (Mhm) and 'Candidatus Mycoplasma turicensis' (Mtc) in cats and their fleas in eastern Australia.

DESIGN AND PROCEDURE

Conventional PCR assays that detect Bartonella spp., M. haemofelis, Mhm, Mtc, Rickettsia spp., Ehrlichia spp., Anaplasma spp. and Neorickettsia spp. were performed on DNA extracted from blood and fleas collected from 111 cats. Cat sera were assayed by ELISA for IgG of Bartonella spp.

RESULTS

DNA of M. haemofelis, Mtc and Mhm was amplified from 1 (0.9%), 1 (0.9%) and 17 cats (15.3%), respectively. Only DNA of Mhm was amplified from the 62 of 111 pooled flea samples (flea sets; 55.9%). Overall, the prevalence rates for Bartonella spp. DNA in the cats and the flea sets was 16.2% (18 cats) and 28.8% (32 flea sets), respectively. Bartonella spp. IgG was detected in 42 cats (37.8%), of which 11 (26.2%) were positive for Bartonella spp. DNA in their blood. R. felis DNA was amplified from 22 flea sets (19.8%), but not from cats. Overall, DNA of one or more of the organisms was amplified from 27% (30) of cats and 67.6% (75) of the flea sets.

CONCLUSIONS

This is the first Australian study to determine the prevalence of R. felis and B. clarridgeiae in both fleas and the cats from which they were collected. Flea-associated infectious agents are common in cats and fleas in eastern Australia and support the recommendation that stringent flea control be maintained on cats.

摘要

目的

确定澳大利亚东部猫及其跳蚤中巴通体属、猫立克次体、溶血支原体、“嗜吞噬细胞无形体”（Mhm）和“图氏无形体”（Mtc）的感染率。

设计与方法

采用常规PCR检测法，对从111只猫采集的血液和跳蚤中提取的DNA进行检测，以检测巴通体属、溶血支原体、Mhm、Mtc、立克次体属、埃立克体属、无形体属和新立克次体属。采用ELISA法检测猫血清中巴通体属的IgG。

结果

分别从1只猫（0.9%）、1只猫（0.9%）和17只猫（15.3%）中扩增出溶血支原体、Mtc和Mhm的DNA。在111份合并跳蚤样本（跳蚤组）中的62份（55.9%）中仅扩增出Mhm的DNA。总体而言，猫和跳蚤组中巴通体属DNA的感染率分别为16.2%（18只猫）和28.8%（32个跳蚤组）。在42只猫（37.8%）中检测到巴通体属IgG，其中11只猫（26.2%）血液中巴通体属DNA呈阳性。从22个跳蚤组（19.8%）中扩增出猫立克次体DNA，但未从猫中扩增出。总体而言，27%（30只）的猫和67.6%（75个）的跳蚤组中扩增出一种或多种病原体的DNA。