Abe Yasuhiko, Hiasa Kyou, Takeuchi Maho, Yoshida Yasuhiro, Suzuki Kazuomi, Akagawa Yasumasa
Department of Advanced Prosthodontics, Division of Cervico-Gnathostomatology, Graduate School of Biomedical Sciences, Hiroshima University, Minami-ku, Japan.
Dent Mater J. 2005 Dec;24(4):536-40. doi: 10.4012/dmj.24.536.
The purpose of this study was to investigate a new biochemical surface modification technique for titanium implants using phospho-amino acid. Pure titanium disks were pretreated with 10 N HCl and ultrapure water at room temperature for 30 minutes respectively. Then these disks were modified with either L-threonine (Thr) or O-phospho-L-threonine (P-Thr) at 37 degrees C for 12 hours. X-ray photoelectron spectroscopy (XPS) chemically analyzed the modified surfaces. It was revealed that the N 1s peak which originated from Thr was not detected in the wide-scan spectrum of Thr-modified surface, whereas three peaks of N 1s, P 2s, and P 2p which originated from P-Thr were detected in the wide-scan spectrum of P-Thr-modified surface. Moreover, the P 2p peak of P-Thr which reacted with the surface significantly shifted to a lower binding energy (p < 0.05). Based on the results of this study, it was concluded that P-Thr chemically bonded to the titanium surface treated with HCl.
本研究的目的是探究一种使用磷酸氨基酸对钛植入物进行生化表面改性的新技术。将纯钛圆盘分别在室温下用10N盐酸和超纯水预处理30分钟。然后将这些圆盘在37℃下用L-苏氨酸（Thr）或O-磷酸-L-苏氨酸（P-Thr）改性12小时。用X射线光电子能谱（XPS）对改性表面进行化学分析。结果显示，在Thr改性表面的宽扫描光谱中未检测到源自Thr的N 1s峰，而在P-Thr改性表面的宽扫描光谱中检测到了源自P-Thr的N 1s、P 2s和P 2p三个峰。此外，与表面反应的P-Thr的P 2p峰显著向较低结合能位移（p < 0.05）。基于本研究结果，得出结论：P-Thr与经盐酸处理的钛表面发生了化学键合。
