Dattelbaum Andrew M, Iyer Srinivas
Materials Science & Technology Division, Los Alamos National Laboratory, Los Alamos, NM 87545, USA.
Expert Rev Proteomics. 2006 Feb;3(1):153-61. doi: 10.1586/14789450.3.1.153.
Laser desorption/ionization mass spectrometry (MS) is rapidly growing in popularity as an analytical characterization method in several fields. The technique shot to prominence using matrix-assisted desorption/ionization for large biomolecules (>700 Da), such as proteins, peptides and nucleic acids. However, because the matrix, which consists of small organic molecules, is also ionized, the technique is of limited use in the low-molecular-mass range (<700 Da). Recent advances in surface science have facilitated the development of matrix-free laser desorption/ionization MS approaches, which are referred to here as surface-assisted laser desorption/ionization (SALDI) MS. In contrast to traditional matrix-assisted techniques, the materials used for SALDI-MS are not ionized, which expands the usefulness of this technique to small-molecule analyses. This review discusses the current status of SALDI-MS as a standard analytical technique, with an emphasis on potential applications in proteomics.
激光解吸/电离质谱法（MS）作为一种分析表征方法在多个领域正迅速受到欢迎。该技术通过基质辅助解吸/电离用于大生物分子（>700 Da），如蛋白质、肽和核酸，从而声名远扬。然而，由于由小分子组成的基质也会被电离，该技术在低分子量范围（<700 Da）的应用有限。表面科学的最新进展推动了无基质激光解吸/电离质谱方法的发展，这里将其称为表面辅助激光解吸/电离（SALDI）质谱法。与传统的基质辅助技术不同，用于SALDI-MS的材料不会被电离，这将该技术的用途扩展到小分子分析。本综述讨论了SALDI-MS作为一种标准分析技术的现状，重点是其在蛋白质组学中的潜在应用。
