Minton K, Durphy M, Taylor R, Friedberg E C
J Biol Chem. 1975 Apr 25;250(8):2823-9.
The T4 ultraviolet endonuclease was previously shown to produce strand incisions (nicks) in ultraviolet-irradiated DNA on the 5' side of thymine dimers. The present studies demonstrate that the purified endonuclease creates 3'-OH and 5'-P termini at the sites of nicking. Photoreactivation of ultraviolet-sensitive sites, thereby demonstrating directly endonucleause has a molecular weight of approximately 18,000 and attacks ultraviolet-irradiated single-stranded Escherichia coli and M-13 DNA.
先前已表明，T4紫外线内切核酸酶可在紫外线照射的DNA中胸腺嘧啶二聚体的5'侧产生链切口（缺口）。目前的研究表明，纯化的内切核酸酶在切口位点产生3'-OH和5'-P末端。紫外线敏感位点的光复活，从而直接证明内切核酸酶的分子量约为18,000，并可作用于紫外线照射的单链大肠杆菌和M-13 DNA。
