Radman M
Basic Life Sci. 1975;5A:197-200. doi: 10.1007/978-1-4684-2895-7_24.
An endonuclease that makes single polynucleotide chain scissions in UV-irradiated DNA has been purified from Escherichia coli. The activity has the following properties: (1) unirradiated DNA is attacked very little if at all; (2) single-stranded DNA is not attacked, whether irradiated or not; (3) there is no requirement for divalent cations, and the activity is not affected by addition of EDTA; (4) the pH optimum is approximately 7; (5) the activity is inhibited by 1 M NaCl, single-stranded DNA, transfer RNA, and unirradiated double-stranded DNA; (6) the sedimentation coefficient, S20, W, is approximately 2.6; (7) it is a basic protein. The enzyme is tentatively named E. coli endonuclease III. The physiological function of the endonuclease has not yet been established.
一种能在紫外线照射的DNA中产生单链多核苷酸链断裂的核酸内切酶已从大肠杆菌中纯化出来。该酶活性具有以下特性：（1）未照射的DNA几乎不被攻击（如果有攻击的话也极少）；（2）单链DNA无论是否受到照射均不被攻击；（3）不需要二价阳离子，添加乙二胺四乙酸（EDTA）对其活性没有影响；（4）最适pH约为7；（5）其活性受到1M氯化钠、单链DNA、转移RNA和未照射的双链DNA的抑制；（6）沉降系数S20，W约为2.6；（7）它是一种碱性蛋白质。该酶暂命名为大肠杆菌核酸内切酶III。这种核酸内切酶的生理功能尚未确定。
