Squaiella Carla Cristina, Ananias Renata Zeigler, Mussalem Juliana Sekeres, Braga Eleni Gonçalves, Rodrigues Elaine Guadelupe, Travassos Luiz R, Lopes José Daniel, Longo-Maugéri Ieda Maria
Disciplina de Imunologia, Departamento de Microbiologia, Imunologia e Parasitologia, Universidade Federal de São Paulo--Escola Paulista de Medicina, Rua Botucatu, 862, 4th andar, 04023-900 São Paulo, Brazil.
Immunobiology. 2006;211(1-2):105-16. doi: 10.1016/j.imbio.2005.10.013. Epub 2005 Dec 27.
Among the effects exerted by Propionibacterium acnes, a most relevant one is its capacity to modulate the Th1/Th2 cellular immune response. This effect depends on the induction and activation of antigen presenting cells, mainly dendritic cells (DCs), whose number is increased in the peripheral blood of animals treated with this bacterium. A soluble P. acnes polysaccharide (PS) extract also acts on DCs, modulating a Th1 immune response. These data led us to investigate the role of P. acnes and its soluble PS on murine bone marrow (BM) DCs. Bone marrow cells were analyzed by flow cytometry, showing an increase of stem cells and DCs in P. acnes- or PS-treated animals. Culturing in the presence of granulocyte monocyte colony stimulating factor (GM-CSF) increased the in vitro differentiation and maturation of these cells into BM-derived DCs (CD11c+ and MHC class II+). Maturation of DCs was determined by increased CD80 and CD86 expression, IL-4 and IL-12 production, reduction in phagocytic capacity and increase in the antigen presenting ability to primed or naïve T lymphocytes. These data indicate that P. acnes as well as its PS can modulate BM stem cells, originating mature DCs, which are important mainly at the initial antigen contact.
在痤疮丙酸杆菌所产生的诸多效应中，一个最为相关的效应是其调节Th1/Th2细胞免疫应答的能力。这种效应取决于抗原呈递细胞（主要是树突状细胞，即DCs）的诱导和激活，在用该细菌处理的动物外周血中，这些细胞的数量会增加。一种可溶性痤疮丙酸杆菌多糖（PS）提取物也作用于DCs，调节Th1免疫应答。这些数据促使我们研究痤疮丙酸杆菌及其可溶性PS在小鼠骨髓（BM）DCs上的作用。通过流式细胞术分析骨髓细胞，结果显示在经痤疮丙酸杆菌或PS处理的动物中干细胞和DCs数量增加。在粒细胞-单核细胞集落刺激因子（GM-CSF）存在的情况下进行培养，可增加这些细胞在体外分化并成熟为BM来源的DCs（CD11c+和MHC II类+）。DCs的成熟通过CD80和CD86表达增加、IL-4和IL-12产生、吞噬能力降低以及对致敏或未致敏T淋巴细胞的抗原呈递能力增强来确定。这些数据表明，痤疮丙酸杆菌及其PS均可调节BM干细胞，产生成熟的DCs，而这些DCs主要在初始抗原接触时发挥重要作用。
