Traum Avram Z, Wells Meghan P, Aivado Manuel, Libermann Towia A, Ramoni Marco F, Schachter Asher D
Division of Nephrology, Children's Hospital Boston, Boston, MA, USA.
Proteomics. 2006 Mar;6(5):1676-80. doi: 10.1002/pmic.200500174.
Proteomic profiling with SELDI-TOF MS has facilitated the discovery of disease-specific protein profiles. However, multicenter studies are often hindered by the logistics required for prompt deep-freezing of samples in liquid nitrogen or dry ice within the clinic setting prior to shipping. We report high concordance between MS profiles within sets of quadruplicate split urine and serum samples deep-frozen at 0, 2, 6, and 24 h after sample collection. Gage R&R results confirm that deep-freezing times are not a statistically significant source of SELDI-TOF MS variability for either blood or urine.
使用表面增强激光解吸电离飞行时间质谱（SELDI-TOF MS）进行蛋白质组分析有助于发现疾病特异性蛋白质谱。然而，多中心研究常常受到临床环境中样本在运输前需迅速在液氮或干冰中深度冷冻所需后勤工作的阻碍。我们报告了在样本采集后0、2、6和24小时深度冷冻的四份重复分割尿液和血清样本组内的质谱谱图之间具有高度一致性。量具重复性与再现性（Gage R&R）结果证实，对于血液或尿液而言，深度冷冻时间并非SELDI-TOF MS变异性的统计学显著来源。
