De Bock Muriel, de Seny Dominique, Meuwis Marie-Alice, Chapelle Jean-Paul, Louis Edouard, Malaise Michel, Merville Marie-Paule, Fillet Marianne
Laboratory of Clinical Chemistry, GIGA Research, University of Liège, CHU, B36, 4000 Liège 1, Belgium.
J Biomed Biotechnol. 2010;2010:906082. doi: 10.1155/2010/906082. Epub 2009 Dec 6.
Protein profiling using SELDI-TOF-MS has gained over the past few years an increasing interest in the field of biomarker discovery. The technology presents great potential if some parameters, such as sample handling, SELDI settings, and data analysis, are strictly controlled. Practical considerations to set up a robust and sensitive strategy for biomarker discovery are presented. This paper also reviews biological fluids generally available including a description of their peculiar properties and the preanalytical challenges inherent to sample collection and storage. Finally, some new insights for biomarker identification and validation challenges are provided.
在过去几年中，使用表面增强激光解吸电离飞行时间质谱（SELDI-TOF-MS）进行蛋白质谱分析在生物标志物发现领域越来越受到关注。如果一些参数，如样品处理、SELDI设置和数据分析等得到严格控制，该技术将展现出巨大潜力。本文提出了建立一个稳健且灵敏的生物标志物发现策略的实际考量。本文还综述了常用的生物体液，包括其独特性质的描述以及样品采集和储存过程中固有的分析前挑战。最后，提供了一些关于生物标志物鉴定和验证挑战的新见解。
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