de Vries G E, Arfman N, Terpstra P, Dijkhuizen L
Department of Microbiology, University of Groningen, Haren, The Netherlands.
J Bacteriol. 1992 Aug;174(16):5346-53. doi: 10.1128/jb.174.16.5346-5353.1992.
The gene (mdh) coding for methanol dehydrogenase (MDH) of thermotolerant, methylotroph Bacillus methanolicus C1 has been cloned and sequenced. The deduced amino acid sequence of the mdh gene exhibited similarity to those of five other alcohol dehydrogenase (type III) enzymes, which are distinct from the long-chain zinc-containing (type I) or short-chain zinc-lacking (type II) enzymes. Highly efficient expression of the mdh gene in Escherichia coli was probably driven from its own promoter sequence. After purification of MDH from E. coli, the kinetic and biochemical properties of the enzyme were investigated. The physiological effect of MDH synthesis in E. coli and the role of conserved sequence patterns in type III alcohol dehydrogenases have been analyzed and are discussed.
嗜热甲基营养型甲醇芽孢杆菌C1中编码甲醇脱氢酶（MDH）的基因（mdh）已被克隆并测序。mdh基因推导的氨基酸序列与其他五种醇脱氢酶（III型）的序列具有相似性，这五种酶不同于含长链锌的（I型）或不含短链锌的（II型）酶。mdh基因在大肠杆菌中的高效表达可能是由其自身的启动子序列驱动的。从大肠杆菌中纯化出MDH后，对该酶的动力学和生化特性进行了研究。分析并讨论了MDH在大肠杆菌中合成的生理效应以及III型醇脱氢酶中保守序列模式的作用。
