与DNA弯曲相关的序列分布在大肠杆菌强启动子的上游被发现。
Sequence distributions associated with DNA curvature are found upstream of strong E. coli promoters.
作者信息
Plaskon R R, Wartell R M
出版信息
Nucleic Acids Res. 1987 Jan 26;15(2):785-96. doi: 10.1093/nar/15.2.785.
The regions upstream from forty-three procaryotic promoters were examined for nucleotide distributions which have been associated with DNA curvature. The analysis procedure assigned a DNA curvature score based on the phasing of the 5' and 3' ends of An and Tn tracts, n greater than or equal to 3. The weighting scheme for the curvature score was based on recent studies which showed that tracts of An and Tn periodically phased with the helix repeat cause DNA curvature. Results show that promoters which have high transcription initiation rates in vivo tend to have high curvature scores in their upstream regions. Regions downstream from the transcription start-point do not have sequences correlated with DNA curvature. Four promoters which have been shown to have upstream activation regions have curvature scores above 1.5 in their -40 to -150 regions. The correlations observed lend support to the hypothesis that DNA curvature is associated with upstream activation of transcription.
对43个原核启动子上游区域进行了核苷酸分布检查，这些分布与DNA弯曲有关。分析程序根据An和Tn序列（n大于或等于3）的5'和3'末端的相位分配DNA弯曲分数。弯曲分数的加权方案基于最近的研究，该研究表明与螺旋重复周期性相位的An和Tn序列会导致DNA弯曲。结果表明，在体内具有高转录起始率的启动子在其上游区域往往具有高弯曲分数。转录起始点下游区域没有与DNA弯曲相关的序列。四个已被证明具有上游激活区域的启动子在其-40至-150区域的弯曲分数高于1.5。观察到的相关性支持了DNA弯曲与转录上游激活相关的假设。
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