[抗双链DNA抗体在胶原涂层上的化学偶联]

[Chemical coupling of anti-dNA antibody on collagen coating].

作者信息

Liu Lan-xia, Leng Xi-gang, Yue Jing-yin, Song Li-ping, Wang Hai, Song Cun-xian

机构信息

Key Laboratory of Biomaterials, Institute of Biomedical Engineering, CAMS and PUMC, Tianjin 300192, China.

出版信息

Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 2005 Dec;27(6):718-22.

PMID:16447645

Abstract

OBJECTIVE

To evaluate the feasibility and stability of chemically conjugating IgM on collagen films.

METHODS

IgM was labeled with 125I using the chloramine-T method. Six collagen films were randomly divided into two groups. In chemical coupling group 125I-labeled IgM was chemically coupled with the films through N-succinmiclyl-3- (2-pyridyl-dithio) propionate reaction. In control group 125I-labeled IgM was absorbed onto collagen films. The amount of IgM on the collagen films and the amount of IgM remained on the films after extensive rinsing with phosphate buffered saline were monitored by counting the radioactivity of 125I.

RESULTS

The amount of antibodies loaded onto collagen films in the chemical coupling group was 15 times higher than that on the control films, showing significant statistical difference (P < 0.01). And the stability of conjugation antibodies on collagen films was significantly better than the control films.

CONCLUSION

Chemical coupling is an effective approach to immobilize antibodies on collagen for further plasmid DNA tethering.

摘要

目的

评估将免疫球蛋白M（IgM）化学偶联到胶原膜上的可行性和稳定性。

方法

采用氯胺-T法用125I标记IgM。将六张胶原膜随机分为两组。化学偶联组通过N-琥珀酰亚胺基-3-（2-吡啶二硫代）丙酸酯反应将125I标记的IgM与膜进行化学偶联。对照组将125I标记的IgM吸附到胶原膜上。通过计数125I的放射性来监测胶原膜上IgM的量以及用磷酸盐缓冲盐水大量冲洗后膜上剩余的IgM量。