Zhang Dan, Zhang Jian-jun
Department of Pharmacology, Institute of Materia Medica, CAMS and PUMC, Beijing 100050, China.
Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 2005 Dec;27(6):729-33.
To study the anti-aging effects of Coeloglossum. viride (L) Hartm. var. bracteatum (Willd) Richter extract (CE) on senescent model mice induced by D-galactose and sodium nitrite.
After one week of accommodation, 60 female NIH mice were divided into six groups with 10 mice in each group: normal control group, aging model group, Piracetam (positive control) group [300 mg/ (kg x d)], and CE reatment groups [2.5, 5, and 10 mg/ (kg x d)]. Mice in aging model group, Piracetam group, and CE treatment group were consecutively intraperitoneally injected with D-galactose [120 mg/ (kg x d)] and sodium nitrite [90 mg/ (kg x d)] for 60 days. From day 47, mice in Piracetam group and CE treatment group were po given Piracetam 300 mg/ (kg x d) or CE 2.5, 5, and 10 mg/ (kg x d). Mice in normal control group and aging model group were po given saline. The drug administration lasted for 14 days. Water maze test was performed to evaluate the learning and memory function in the mice. The activity of superoxide dismutase (SOD), the content of malondialdehyde (MDA), and the activities of adenosinetriphosphatase (ATPase), and monoamine-oxidase (MAO) in brain tissue were measured.
The latencies in water maze test in aging model group mice were significantly longer than in normal control group (P < 0.01, P < 0.05), and the number of errors increased (P < 0.05). In aging model group mice, the activities of SOD, Na+K(+)-ATPase, and Ca2+Mg(2+)-ATPase decreased (P < 0.01, P < 0.05), while the content of MDA and the activities of MAO-A and MAO-B increased (P < 0.01). Piracetam [300 mg/ (kg x d), po] and CE [2.5, 5, 10 mg/ (kg x d), po] ameliorated the above changes in aging model mice.
CE may improve the memory dysfunction induced by consecutive injection of D-galactose and sodium nitrite,and has nootropic and antiaging effects.
研究绶草提取物（CE）对D-半乳糖和亚硝酸钠诱导的衰老模型小鼠的抗衰老作用。
适应性饲养1周后，将60只雌性NIH小鼠分为6组，每组10只：正常对照组、衰老模型组、吡拉西坦（阳性对照）组[300mg/（kg·d）]和CE治疗组[2.5、5和10mg/（kg·d）]。衰老模型组、吡拉西坦组和CE治疗组小鼠连续腹腔注射D-半乳糖[120mg/（kg·d）]和亚硝酸钠[90mg/（kg·d）]60天。从第47天起，吡拉西坦组和CE治疗组小鼠灌胃给予吡拉西坦300mg/（kg·d）或CE 2.5、5和10mg/（kg·d）。正常对照组和衰老模型组小鼠灌胃给予生理盐水。给药持续14天。进行水迷宫试验以评估小鼠的学习和记忆功能。测定脑组织中超氧化物歧化酶（SOD）活性、丙二醛（MDA）含量、腺苷三磷酸酶（ATPase）和单胺氧化酶（MAO）活性。
衰老模型组小鼠水迷宫试验中的潜伏期显著长于正常对照组（P<0.01，P<0.05），错误次数增加（P<0.05）。衰老模型组小鼠SOD、Na+K（+）-ATPase和Ca2+Mg（2+）-ATPase活性降低（P<0.01，P<0.05），而MDA含量以及MAO-A和MAO-B活性增加（P<0.01）。吡拉西坦[300mg/（kg·d），灌胃]和CE[2.5、5、10mg/（kg·d），灌胃]改善了衰老模型小鼠的上述变化。
CE可能改善连续注射D-半乳糖和亚硝酸钠所致的记忆功能障碍，具有益智和抗衰老作用。
