Zhang Dan, Liu Geng-Tao, Shi Jian-Gong, Zhang Jian-Jun
Department of Pharmacology, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.
Basic Clin Pharmacol Toxicol. 2006 Jan;98(1):55-60. doi: 10.1111/j.1742-7843.2006.pto_218.x.
Previous studies have shown that injection of D-galactose could result in senescent performances in animals, that injection of NaNO2 could cause ischaemia and hypoxia in many organs, and combined injection of D-galactose and NaNO2 make normal mice taking on senescent performances in a shorter period. The aim of this study was to investigate the effects of CE, an extract from a Tibetan medicinal herb, Coeloglossum. viride (L.) Hartm. var. bracteatum (Willd.), on senescent mice. The step-down test was performed to evaluate the learning and memory function of mice. The activities of superoxide dismutase, adenosine triphosphatase, monoamine oxydase and the content of malondialdehyde were measured to determine the impairment of brain. The expressions of Bcl-2, Bax, and caspase-3 proteins in mouse hippocampus were studied by immunohistochemical staining. The data demonstrated that D-galactose and NaNO2 treated mice had significant deficits in learning and memory function. The reduced activities of superoxide dismutase, adenosine triphosphatase, increased activities of monoamine oxydase and level of malondialdehyde were also found. Bax and caspase-3 positive cells increased while Bcl-2 positive cells decreased remarkably. Treatment of CE (2.5, 5 mg.kg(-1)) ameliorated the memory impairment; rectified the biochemistry and neural system changes in mice. These results suggest that CE offers promise as a tool for treatment of senescence-related diseases.
先前的研究表明，注射D-半乳糖可导致动物出现衰老表现，注射亚硝酸钠可使许多器官发生缺血和缺氧，而联合注射D-半乳糖和亚硝酸钠会使正常小鼠在更短时间内呈现衰老表现。本研究的目的是探讨藏药绿花舌唇兰（Coeloglossum viride (L.) Hartm. var. bracteatum (Willd.)）提取物CE对衰老小鼠的影响。采用跳台试验评估小鼠的学习和记忆功能。测定超氧化物歧化酶、三磷酸腺苷酶、单胺氧化酶的活性以及丙二醛的含量，以确定脑损伤情况。通过免疫组织化学染色研究小鼠海马中Bcl-2、Bax和caspase-3蛋白的表达。数据表明，用D-半乳糖和亚硝酸钠处理的小鼠在学习和记忆功能方面存在显著缺陷。还发现超氧化物歧化酶、三磷酸腺苷酶的活性降低，单胺氧化酶的活性和丙二醛水平升高。Bax和caspase-3阳性细胞增加，而Bcl-2阳性细胞显著减少。CE（2.5、5 mg·kg⁻¹）治疗改善了记忆障碍；纠正了小鼠的生化和神经系统变化。这些结果表明，CE有望成为治疗衰老相关疾病的一种手段。
