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枯草芽孢杆菌168染色体上II型DNA与膜结合位点和oriC之间的物理距离。

Physical distance between the site of type II DNA binding to the membrane and oriC on the Bacillus subtilis 168 chromosome.

作者信息

Itaya M, Laffan J J, Sueoka N

机构信息

Mitsubishi Kasei Institute of Life Sciences, Tokyo, Japan.

出版信息

J Bacteriol. 1992 Aug;174(16):5466-70. doi: 10.1128/jb.174.16.5466-5470.1992.

DOI:10.1128/jb.174.16.5466-5470.1992
PMID:1644775
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC206389/
Abstract

The precise physical locations of the oriC region and the region for type II DNA binding to the membrane on the Bacillus subtilis 168 chromosome were determined. The DNA regions were physically mapped by creating new restriction sites (NotI and SfiI) within these regions. The physical distance between oriC and the type II DNA-binding region was verified with the creation of a novel sequence cleaved by endonuclease I-SceI in each of the above regions. Complete removal of the defined type II membrane-binding region produced no noticeable phenotype.

摘要

确定了枯草芽孢杆菌168染色体上oriC区域以及II型DNA与膜结合区域的精确物理位置。通过在这些区域内创建新的限制酶切位点（NotI和SfiI）对DNA区域进行物理图谱绘制。通过在上述每个区域创建一个被I-SceI内切酶切割的新序列，验证了oriC与II型DNA结合区域之间的物理距离。完全去除确定的II型膜结合区域未产生明显的表型。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/11c8/206389/dbdfcb287c9a/jbacter00082-0304-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/11c8/206389/cf941af75c11/jbacter00082-0302-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/11c8/206389/07f0b7e661a2/jbacter00082-0303-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/11c8/206389/dbdfcb287c9a/jbacter00082-0304-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/11c8/206389/cf941af75c11/jbacter00082-0302-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/11c8/206389/07f0b7e661a2/jbacter00082-0303-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/11c8/206389/dbdfcb287c9a/jbacter00082-0304-a.jpg

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Proc Natl Acad Sci U S A. 1997 May 13;94(10):5378-82. doi: 10.1073/pnas.94.10.5378.
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Suppression of initiation defects of chromosome replication in Bacillus subtilis dnaA and oriC-deleted mutants by integration of a plasmid replicon into the chromosomes.通过将质粒复制子整合到染色体中抑制枯草芽孢杆菌dnaA和oriC缺失突变体中染色体复制起始缺陷。
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本文引用的文献

1
Site-specific in vitro binding of plasmid pUB110 to Bacillus subtilis membrane fraction.质粒pUB110与枯草芽孢杆菌膜组分的位点特异性体外结合
J Bacteriol. 1983 Jun;154(3):1184-94. doi: 10.1128/jb.154.3.1184-1194.1983.
2
Amplification of a major membrane-bound DNA sequence of Bacillus subtilis.枯草芽孢杆菌主要膜结合DNA序列的扩增
J Bacteriol. 1985 Feb;161(2):589-95. doi: 10.1128/jb.161.2.589-595.1985.
3
The normal replication terminus of the Bacillus subtilis chromosome, terC, is dispensable for vegetative growth and sporulation.
Stability and asymmetric replication of the Bacillus subtilis 168 chromosome structure.
枯草芽孢杆菌168染色体结构的稳定性与不对称复制。
J Bacteriol. 1993 Feb;175(3):741-9. doi: 10.1128/jb.175.3.741-749.1993.
4
Integration of repeated sequences (pBR322) in the Bacillus subtilis 168 chromosome without affecting the genome structure.重复序列（pBR322）整合到枯草芽孢杆菌168染色体中，而不影响基因组结构。
Mol Gen Genet. 1993 Nov;241(3-4):287-97. doi: 10.1007/BF00284680.
5
Toward a bacterial genome technology: integration of the Escherichia coli prophage lambda genome into the Bacillus subtilis 168 chromosome.迈向细菌基因组技术：将大肠杆菌噬菌体λ基因组整合到枯草芽孢杆菌168染色体中。
Mol Gen Genet. 1995 Jul 22;248(1):9-16. doi: 10.1007/BF02456608.
枯草芽孢杆菌染色体的正常复制终点terC，对于营养生长和孢子形成来说并非必需。
J Mol Biol. 1987 May 20;195(2):299-310. doi: 10.1016/0022-2836(87)90651-6.
4
Identification of a specific membrane-particle-associated DNA sequence in Bacillus subtilis.枯草芽孢杆菌中一种特定的膜颗粒相关DNA序列的鉴定。
J Bacteriol. 1986 Apr;166(1):38-43. doi: 10.1128/jb.166.1.38-43.1986.
5
Revised genetic linkage map of Bacillus subtilis.枯草芽孢杆菌的修订遗传连锁图谱。
Microbiol Rev. 1985 Jun;49(2):158-79. doi: 10.1128/mr.49.2.158-179.1985.
6
Structure and function of the region of the replication origin of the Bacillus subtilis chromosome. IV. Transcription of the oriC region and expression of DNA gyrase genes and other open reading frames.枯草芽孢杆菌染色体复制起点区域的结构与功能。IV. oriC区域的转录以及DNA促旋酶基因和其他开放阅读框的表达。
Nucleic Acids Res. 1985 Apr 11;13(7):2267-79. doi: 10.1093/nar/13.7.2267.
7
Structure and function of the region of the replication origin of the Bacillus subtilis chromosome. III. Nucleotide sequence of some 10,000 base pairs in the origin region.枯草芽孢杆菌染色体复制起点区域的结构与功能。III. 起点区域约10,000个碱基对的核苷酸序列。
Nucleic Acids Res. 1985 Apr 11;13(7):2251-65. doi: 10.1093/nar/13.7.2251.
8
A neomycin resistance gene cassette selectable in a single copy state in the Bacillus subtilis chromosome.一种在枯草芽孢杆菌染色体中以单拷贝状态可选择的新霉素抗性基因盒。
Nucleic Acids Res. 1989 Jun 12;17(11):4410. doi: 10.1093/nar/17.11.4410.
9
The blasticidin S resistance gene (bsr) selectable in a single copy state in the Bacillus subtilis chromosome.杀稻瘟菌素S抗性基因（bsr）在枯草芽孢杆菌染色体中以单拷贝状态可选择。
J Biochem. 1990 Jun;107(6):799-801. doi: 10.1093/oxfordjournals.jbchem.a123128.
10
Cleavage of yeast and bacteriophage T7 genomes at a single site using the rare cutter endonuclease I-Sce I.使用稀有切割内切核酸酶I-Sce I在单个位点切割酵母和噬菌体T7基因组。
Nucleic Acids Res. 1991 Jan 11;19(1):189-90. doi: 10.1093/nar/19.1.189.