Sato Y, McCollum M, McKenzie T, Laffan J, Zuberi A, Sueoka N
Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder 80309-0347.
J Bacteriol. 1991 Dec;173(23):7732-5. doi: 10.1128/jb.173.23.7732-7735.1991.
DNA-membrane association critical for initiation of DNA replication in Bacillus subtilis can be classified into two types. Type I is salt resistant and dependent on the initiation gene, dnaB, and type II is salt sensitive and independent of the dnaB gene. We found and sequenced two adjacent areas of type II binding within 1% of oriC on the B. subtilis chromosome.
对枯草芽孢杆菌中DNA复制起始至关重要的DNA-膜结合可分为两种类型。I型耐盐且依赖于起始基因dnaB,II型对盐敏感且不依赖于dnaB基因。我们在枯草芽孢杆菌染色体上oriC的1%范围内发现并测序了II型结合的两个相邻区域。