使用微流控技术的并行皮升逆转录聚合酶链反应检测

Parallel picoliter rt-PCR assays using microfluidics.

作者信息

Marcus Joshua S, Anderson W French, Quake Stephen R

机构信息

Option in Biochemistry and Molecular Biophysics, Department of Applied Physics, California Institute of Technology, Pasadena, 91125, USA.

出版信息

Anal Chem. 2006 Feb 1;78(3):956-8. doi: 10.1021/ac0513865.

DOI:10.1021/ac0513865

PMID:16448074

Abstract

The development of microfluidic tools for high-throughput nucleic acid analysis has become a burgeoning area of research in the post-genome era. Here, we have developed a microfluidic chip to perform 72 parallel 450-pL RT-PCRs. We took advantage of Taqman hydrolysis probe chemistry to detect RNA templates as low as 34 copies. The device and method presented here may enable highly parallel single cell gene expression analysis.

摘要

用于高通量核酸分析的微流控工具的开发已成为后基因组时代一个蓬勃发展的研究领域。在此，我们开发了一种微流控芯片来进行72个平行的450皮升逆转录聚合酶链反应（RT-PCR）。我们利用Taqman水解探针化学方法来检测低至34个拷贝的RNA模板。本文介绍的设备和方法可能实现高度平行的单细胞基因表达分析。