Alanko Lauri, Porkka-Heiskanen Tarja, Soinila Seppo
Department of Physiology, Institute of Biomedicine, University of Helsinki, Finland.
J Chem Neuroanat. 2006 Apr;31(3):162-8. doi: 10.1016/j.jchemneu.2005.12.001. Epub 2006 Jan 31.
Adenosine is a recognized inhibitory neuromodulator and neuroprotective agent in the central nervous system. It is produced both intra- and extracellularly and transported across the cell membrane. Nucleoside transporters thus have a major impact on the extracellular adenosine levels, and consequently adenosine signalling. We have raised and characterized polyclonal antibodies against both the equilibrative nucleoside transporters 1 and 2, and report for the first time their distribution in rat brain at the cellular level. Double staining studies were performed to assess the localization of the transporters in neural and glial cells. Both transporters were present in practically all neurons. Some astrocytes showed equilibrative nucleoside transporter 1 staining, while equilibrative nucleoside transporter 2 staining on astrocytes was observed only sporadically.
腺苷是中枢神经系统中一种公认的抑制性神经调质和神经保护剂。它在细胞内和细胞外均有产生，并跨细胞膜运输。核苷转运体因此对细胞外腺苷水平以及腺苷信号传导有重大影响。我们制备并鉴定了针对平衡核苷转运体1和2的多克隆抗体，并首次在细胞水平上报告了它们在大鼠脑中的分布。进行了双重染色研究以评估转运体在神经细胞和神经胶质细胞中的定位。两种转运体几乎存在于所有神经元中。一些星形胶质细胞显示出平衡核苷转运体1染色，而星形胶质细胞上的平衡核苷转运体2染色仅偶尔观察到。
