Sumathy S, Thyagarajan S P, Latif R, Madanagopalan N, Raguram K, Rajasambandam P, Gowans E
Department of Microbiology, Dr. A. L. M. Post Graduate Institute of Basic Medical Sciences, Taramani, Madras, India.
J Virol Methods. 1992 Jul;38(1):145-52. doi: 10.1016/0166-0934(92)90177-f.
A simple, specific and economical dipstick immunobinding enzyme-linked immunosorbent assay (DIA) for detecting hepatitis B surface antigen (HBsAg) and antibodies to hepatitis delta virus (anti-HDV), utilizing cellulose nitrate membrane is described. Screening of 815 serum specimens for HBsAg by DIA and micro ELISA revealed a positivity of 22.69% and 22.94% respectively. In the detection of antibodies to delta antigen, DIA was compared with an indirect immunofluorescence technique using A3 cell line as antigen substrate and a commercial macro ELISA. Of the 143 HBsAg positive sera tested for anti-HDV, 59 (41.25%) were positive by both immunofluorescence and macro ELISA and 61 (42.65%) by DIA. While the positive and negative predictive values of DIA for HBsAg were 100% and 99.6%, for anti-HDV by DIA these were 96.7% and 100% respectively. Based on the simplicity of performance and the economical nature of the test system, DIA is recommended as a diagnostic tool for field surveys and small laboratories in developing countries.
本文描述了一种利用硝酸纤维素膜检测乙型肝炎表面抗原（HBsAg）和丁型肝炎病毒抗体（抗-HDV）的简单、特异且经济的试纸条免疫结合酶联免疫吸附测定（DIA）方法。通过DIA和微量ELISA对815份血清标本进行HBsAg筛查，阳性率分别为22.69%和22.94%。在检测抗-HDV时，将DIA与以A3细胞系为抗原底物的间接免疫荧光技术及一种市售的微量ELISA进行了比较。在检测的143份HBsAg阳性血清中，59份（41.25%）通过免疫荧光和微量ELISA检测均为阳性，61份（42.65%）通过DIA检测为阳性。DIA检测HBsAg的阳性预测值和阴性预测值分别为100%和99.6%，检测抗-HDV的阳性预测值和阴性预测值分别为96.7%和100%。基于操作的简便性和检测系统的经济性，DIA被推荐作为发展中国家现场调查和小型实验室的诊断工具。
