Carrari Fernando, Fernie Alisdair R
Instituto de Biotecnología, CICVyA, Instituto Nacional de Tecnología Agrícola (IB-INTA) Argentina.
J Exp Bot. 2006;57(9):1883-97. doi: 10.1093/jxb/erj020. Epub 2006 Jan 31.
The development and maturation of tomato fruits has received considerable attention because of both the uniqueness of such processes to the biology of plants and the importance of these fruits as a component of the human diet. Molecular and genetic analysis of fruit development, and especially ripening of fleshy fruits, has resulted in significant gains in knowledge over recent years. A large amount of knowledge has been gathered on ethylene biosynthesis and response, cell wall metabolism, and environmental factors, such as light, that impact ripening. Considerably less attention has been paid directly to the general metabolic shifts that underpin these responses. Given the vast complexity of fruit metabolism, the focus chosen for this review is on primary metabolites and those secondary metabolites that are important with respect to fruit quality. Here, recent advances in dissecting tomato metabolic pathways are reviewed. Also discussed are recent examples in which the combined application of metabolic and transcriptional profiling, aimed at identifying candidate genes for modifying metabolite contents, was used.
番茄果实的发育和成熟受到了广泛关注，这既是因为这些过程在植物生物学中的独特性，也是因为这些果实作为人类饮食组成部分的重要性。近年来，对果实发育，尤其是肉质果实成熟的分子和遗传分析取得了显著的知识进展。关于乙烯生物合成与反应、细胞壁代谢以及影响成熟的环境因素（如光照），已经积累了大量知识。然而，对于支撑这些反应的一般代谢变化，直接关注的程度要低得多。鉴于果实代谢的极其复杂性，本综述选择关注的重点是初级代谢产物以及那些对果实品质重要的次级代谢产物。在此，将对剖析番茄代谢途径的最新进展进行综述。还将讨论近期一些实例，其中运用了代谢和转录谱分析的联合应用，旨在鉴定用于修饰代谢物含量的候选基因。
