Franz Charles M A P, Vancanneyt Marc, Vandemeulebroecke Katrien, De Wachter Marjan, Cleenwerck Ilse, Hoste Bart, Schillinger Ulrich, Holzapfel Wilhelm H, Swings Jean
Federal Research Centre for Nutrition and Food, Institute for Hygiene and Toxicology, Haid-und-Neu-Strasse 9, D-76131 Karlsruhe, Germany.
BCCM/LMG Bacteria Collection, Laboratory of Microbiology, Ghent University, Ledeganckstraat 35, B-9000 Ghent, Belgium.
Int J Syst Evol Microbiol. 2006 Feb;56(Pt 2):329-333. doi: 10.1099/ijs.0.63944-0.
A Gram-positive, coccus-shaped, lactic acid bacterium, strain LMG 23082T, was isolated from steeped maize grains. The organism is homofermentative and produces D- and L-lactic acid from glucose. 16S rRNA gene sequence analysis revealed that the organism belongs to the genus Pediococcus, with Pediococcus pentosaceus and Pediococcus acidilactici as nearest neighbours. Genotypic fingerprinting, whole-cell protein electrophoresis, DNA-DNA hybridizations and physiological and biochemical tests allowed differentiation of strain LMG 23082T from other established Pediococcus species. A remarkable feature was that, unlike other pediococci, this bacterium was capable of growth at pH 9.0. The strain studied represents a novel species for which the name Pediococcus stilesii sp. nov. is proposed with the type strain LMG 23082T (=BFE 1652T=FAIR-E 180T=CCUG 51290T), the only currently known isolate of the species.
从浸泡过的玉米粒中分离出一株革兰氏阳性、球菌状的乳酸菌，菌株LMG 23082T。该菌为同型发酵菌，可利用葡萄糖产生D-型和L-型乳酸。16S rRNA基因序列分析表明，该菌属于片球菌属，与戊糖片球菌和嗜酸片球菌亲缘关系最近。通过基因型指纹图谱、全细胞蛋白质电泳、DNA-DNA杂交以及生理生化试验，可将菌株LMG 23082T与其他已确定的片球菌属物种区分开来。一个显著特征是，与其他片球菌不同，该菌能够在pH 9.0条件下生长。所研究的菌株代表了一个新物种，为此提出新名称嗜碱片球菌（Pediococcus stilesii sp. nov.），模式菌株为LMG 23082T（=BFE 1652T=FAIR-E 180T=CCUG 51290T），这是目前已知的该物种的唯一分离株。
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