Brady Carrie L, Venter Stephanus N, Cleenwerck Ilse, Engelbeen Katrien, Vancanneyt Marc, Swings Jean, Coutinho Teresa A
Department of Microbiology and Plant Pathology, Forestry and Agricultural Biotechnology Institute (FABI), University of Pretoria, Pretoria 0002, South Africa.
Int J Syst Evol Microbiol. 2009 Sep;59(Pt 9):2339-45. doi: 10.1099/ijs.0.009241-0. Epub 2009 Jul 20.
Bacteria isolated from eucalyptus leaves and shoots showing symptoms of blight and die-back collected in Uganda, Uruguay and Argentina and from maize displaying brown stalk rot symptoms in South Africa were tentatively placed in the genus Pantoea on the basis of phenotypic and biochemical tests. These isolates, together with two strains (LMG 2558 and LMG 2560) previously assigned to Pantoea agglomerans based on protein electrophoregrams but later excluded from this species, were further investigated using molecular techniques. 16S rRNA gene sequencing and multilocus sequence analyses (MLSA) revealed that the strains were phylogenetically closely related to Pantoea agglomerans, Pantoea stewartii and Pantoea ananatis. MLSA and amplified fragment length polymorphism analysis placed the strains into four separate clusters, not containing any of the type strains of species of the genus Pantoea. DNA-DNA hybridization confirmed the classification of the isolates into four novel species, for which the names Pantoea vagans sp. nov. (type strain R-21566T=LMG 24199T=BCC 105T=BD 765T), Pantoea eucalypti sp. nov. (type strain R-25678T=LMG 24197T=BCC 076T=BD 769T), Pantoea deleyi sp. nov. (type strain R-31523T=LMG 24200T=BCC 109T=BD 767T) and Pantoea anthophila sp. nov. (type strain LMG 2558T=BD 871T=NCPPB 1682T) are proposed.
从乌干达、乌拉圭和阿根廷采集的表现出枯萎和枝梢枯死症状的桉树叶和嫩枝，以及从南非采集的表现出褐色茎腐病症状的玉米中分离出的细菌，基于表型和生化测试，初步被归入泛菌属。这些分离株，连同之前根据蛋白质电泳图谱被归入成团泛菌但后来被排除在该物种之外的两个菌株（LMG 2558和LMG 2560），使用分子技术进行了进一步研究。16S rRNA基因测序和多位点序列分析（MLSA）表明，这些菌株在系统发育上与成团泛菌、斯氏泛菌和菠萝泛菌密切相关。MLSA和扩增片段长度多态性分析将这些菌株分为四个独立的簇，不包含泛菌属任何物种的模式菌株。DNA-DNA杂交证实这些分离株可分为四个新物种，为此提出了新种名称：泛菌属新种漫游泛菌（模式菌株R-21566T = LMG 24199T = BCC 105T = BD 765T）、泛菌属新种桉叶泛菌（模式菌株R-25678T = LMG 24197T = BCC 076T = BD 769T）、泛菌属新种德莱泛菌（模式菌株R-31523T = LMG 24200T = BCC 109T = BD 767T）和泛菌属新种嗜花泛菌（模式菌株LMG 2558T = BD 871T = NCPPB 1682T）。
