Doi Katsumi, Nishizaki Yousuke, Fujino Yasuhiro, Ohshima Toshihisa, Ohmomo Sadahiro, Ogata Seiya
Department of Genetic Resources Technology, Faculty of Agriculture, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan.
Int J Syst Evol Microbiol. 2009 May;59(Pt 5):1007-10. doi: 10.1099/ijs.0.005793-0.
A Gram-positive, coccus-shaped, lactic acid bacterium, strain NGRI 0510Q(T), was isolated from ryegrass silage produced in Okinawa Prefecture, Japan. The cell is non-spore-forming, non-motile, and occurs in pairs or tetrads. The strain is homofermentative and produces d- and l-lactic acid from glucose. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain NGRI 0510Q(T) belongs to the genus Pediococcus and clusters within the Pediococcus acidilactici and Pediococcus pentosaceus group, with 98.2 and 96.9 % sequence identity, respectively. DNA-DNA relatedness between strain NGRI 0510Q(T) and P. acidilactici JCM 8797(T) and P. pentosaceus JCM 5890(T) was 19.3 and 17.3 %, respectively. Based on its phenotypic characteristics, phylogenetic relationship and DNA-DNA relatedness, NGRI 0510Q(T) (=JCM 15055(T)=DSM 19927(T)) represents the type strain of a novel species, for which the name Pediococcus lolii sp. nov. is proposed.
从日本冲绳县生产的黑麦草青贮饲料中分离出一株革兰氏阳性、球菌状的乳酸菌，菌株NGRI 0510Q(T)。该细胞不形成芽孢，无运动性，成对或四联状存在。该菌株为同型发酵菌，能从葡萄糖产生d-和l-乳酸。基于16S rRNA基因序列的系统发育分析表明，菌株NGRI 0510Q(T)属于片球菌属，聚集在嗜酸片球菌和戊糖片球菌组内，序列同一性分别为98.2%和96.9%。菌株NGRI 0510Q(T)与嗜酸片球菌JCM 8797(T)和戊糖片球菌JCM 5890(T)之间的DNA-DNA相关性分别为19.3%和17.3%。基于其表型特征、系统发育关系和DNA-DNA相关性，NGRI 0510Q(T)(=JCM 15055(T)=DSM 19927(T))代表一个新物种的模式菌株，为此提出了新名称戊糖片球菌（Pediococcus lolii sp. nov.）。
