Bockwoldt Julia A, Fellermeier Johanna, Steffens Emma, Vogel Rudi F, Ehrmann Matthias A
Lehrstuhl für Technische Mikrobiologie, Technische Universität München, 85354 Freising, Germany.
Foods. 2021 Mar 6;10(3):547. doi: 10.3390/foods10030547.
Sourdough fermentation is a common practice spread across the globe due to quality and shelf life improvement of baked goods. Above the widely studied exopolysaccharide (EPS) formation, which is exploited for structural improvements of foods including baked goods, β-glucan formation, by using lactic acid bacteria (LAB), offers additional values. Through renunciation of sucrose addition for bacterial β-d-glucan formation, which is required for the production of other homopolysaccharides, residual sweetness of baked goods can be avoided, and predicted prebiotic properties can be exploited. As promising starter cultures () TMW (Technische Mikrobiologie Weihenstephan) 1.2112 and () TMW 2.340 produce 2-substituted (1,3)-β-d-glucan upon fermenting wheat and rye doughs. In this study, we have evaluated methods for bacterial β-glucan quantification, identified parameters influencing the β-glucan yield in fermented sourdoughs, and evaluated the sourdough breads by an untrained sensory panel. An immunological method for the specific detection of β-glucan proved to be suitable for its quantification, and changes in the fermentation temperature were related to higher β-glucan yields in sourdoughs. The sensory analysis resulted in an overall acceptance of the wheat and rye sourdough breads fermented by and with a preference of the fermented wheat sourdough bread and tart-flavored rye sourdough bread.
由于酸面团发酵能够改善烘焙食品的品质和保质期,因此在全球范围内广泛应用。除了对广泛研究的胞外多糖(EPS)形成进行利用,以改善包括烘焙食品在内的食品结构外,利用乳酸菌(LAB)形成β-葡聚糖还具有其他价值。通过放弃添加蔗糖来形成细菌β-d-葡聚糖(这是生产其他同多糖所必需的),可以避免烘焙食品的残留甜味,并利用预测的益生元特性。作为有前景的发酵剂培养物,TMW(魏恩斯泰法恩技术微生物学)1.2112和TMW 2.340在发酵小麦和黑麦面团时会产生2-取代的(1,3)-β-d-葡聚糖。在本研究中,我们评估了细菌β-葡聚糖定量方法,确定了影响发酵酸面团中β-葡聚糖产量的参数,并通过未经训练的感官小组对酸面团面包进行了评估。一种用于特异性检测β-葡聚糖的免疫学方法被证明适用于其定量,发酵温度的变化与酸面团中较高的β-葡聚糖产量相关。感官分析结果表明,由TMW 1.2112和TMW 2.340发酵的小麦和黑麦酸面团面包总体上被接受,其中TMW 1.2112发酵的小麦酸面团面包和酸味黑麦酸面团面包更受青睐。