Keshk Sherif, Sameshima Kazuhiko
Department of Basic Environmental Science, Institute of Environmental Studies and Research, Ain Shams University, Cairo, Abbasia, 11566, Egypt.
Appl Microbiol Biotechnol. 2006 Sep;72(2):291-6. doi: 10.1007/s00253-005-0265-6. Epub 2006 Feb 1.
Production of bacterial cellulose (BC) using sugar cane molasses (MO) with/without the presence of lignosulfonate (MOL) as a sole carbon source in a Hestrin-Schramm medium (HS) was investigated. Six strains of Acetobacter xylinum [American Type Culture Collection 10245 and Institute of Fermentation in Osaka (IFO) 13693, 13772, 13773, 14815, and 15237] were screened for their BC production. The yield of the BC among all the strains from both the MO and MOL media was much higher than that from the HS medium. Acetobacter xylinum IFO 13772 was the best BC producer for all media. Furthermore, physical properties of these BC from the HS, MO, and MOL media were studied using Fourier-transform infrared spectroscopy, X-ray diffractometer, and cross polarization/magic angle spinning 13C nuclear magnetic resonance. There are no significant differences in the crystallinity and the recorded Ialpha fraction among the BC produced from the different media. A remarkable difference was only recorded in terms of viscosity. These results indicate that MO is a better carbon source than glucose for most of the strains investigated.
研究了在赫斯特林-施拉姆培养基(HS)中,以甘蔗废蜜(MO)为唯一碳源,添加/不添加木质素磺酸盐(MOL)来生产细菌纤维素(BC)的情况。筛选了六株木醋杆菌[美国典型培养物保藏中心10245以及大阪发酵研究所(IFO)13693、13772、13773、14815和15237]的BC生产能力。在MO和MOL培养基中所有菌株产生的BC产量都远高于HS培养基中的产量。木醋杆菌IFO 13772是所有培养基中最佳的BC生产者。此外,使用傅里叶变换红外光谱仪、X射线衍射仪和交叉极化/魔角旋转13C核磁共振研究了HS、MO和MOL培养基中这些BC的物理性质。不同培养基生产的BC在结晶度和记录的Iα分数方面没有显著差异。仅在粘度方面记录到显著差异。这些结果表明,对于大多数所研究的菌株而言,MO是比葡萄糖更好的碳源。