Sulaiman Fatah
Chemical Engineering Department, Universitas Sultan Ageng Tirtayasa, Jl. Jendral Sudirman Km 3, Cilegon 42435, Banten, Indonesia.
Center of Excellence Local Food Innovation, Universitas Sultan Ageng Tirtayasa, Jl. Raya Palka Km 3, Sindangsari, Kabupaten Serang 42118, Banten, Indonesia.
Polymers (Basel). 2023 Feb 9;15(4):870. doi: 10.3390/polym15040870.
Wastewater from the Arenga starch industry (WWAS) contains a high chemical oxygen demand (COD) concentration, so it has to be treated before being discharged into water bodies. Therefore, the purpose of this study was to utilize WWAS as a medium for bacterial cellulose (BC) and cellulose acetate (CA) production. This study consisted of the production of BC through fermentation and the production of CA through acetylation. Fermentation was conducted under static batch conditions with various initial pHs and sucrose additions, while acetylation was conducted with various BC-acetic anhydride ratios. The results of this study showed that the maximum BC production of 505.6 g/L of the culture medium was obtained under the optimal conditions of a sucrose addition of 200 g/L, an initial medium pH of 4.5, and a cultivation time of 14 d. Furthermore, a BC-acetic anhydride ratio of 1:3 resulted in CA being suitable as a biofilm raw material with a yield of 81.49%, an acetyl content of 39.82%, a degree of substitution of 2.456, and a degree of crystallinity of 36.7%. FT-IR, H and C NMR, XRD, and SEM analyses confirmed the successful process of acetylation of BC to CA.
槟榔淀粉工业废水（WWAS）的化学需氧量（COD）浓度很高，因此在排放到水体之前必须进行处理。因此，本研究的目的是利用WWAS作为生产细菌纤维素（BC）和醋酸纤维素（CA）的介质。本研究包括通过发酵生产BC和通过乙酰化生产CA。发酵在静态分批条件下进行，改变初始pH值和蔗糖添加量，而乙酰化则采用不同的BC-乙酸酐比例进行。本研究结果表明，在蔗糖添加量为200 g/L、初始培养基pH值为4.5、培养时间为14 d的最佳条件下，培养基中BC的最大产量为505.6 g/L。此外，BC-乙酸酐比例为1:3时，所得CA适合作为生物膜原料，产率为81.49%，乙酰含量为39.82%，取代度为2.456，结晶度为36.7%。傅里叶变换红外光谱（FT-IR）、氢核磁共振（H NMR）和碳核磁共振（C NMR）、X射线衍射（XRD）和扫描电子显微镜（SEM）分析证实了BC成功乙酰化为CA的过程。
