Silva L F, Alves L C, Santos S S, Cavalcanti M G S, Peixoto C A
Departamento de Biologia Celular e Ultraestrutura, Centro de Pesquisas Aggeu Magalhães (FIOCRUZ), Recife, Brazil.
Parasitol Res. 2006 Jun;99(1):14-20. doi: 10.1007/s00436-005-0054-2. Epub 2006 Feb 1.
In the present paper, we report the results we obtained using several cytochemical techniques to analyze the infective larva of Wuchereria bancrofti. An imidazole osmium tetroxide solution was used to visualize unsaturated fatty acids. A highly contrasted material forming a continuous structure was observed on the larval surface and over the epicuticle. A strong reaction was observed on the esophagus and also on the inner secreted material. Carbohydrates containing vic-glycol groups were not observed on the cuticle of the third-stage larva of W. bancrofti submitted to the Thiéry technique. Using a panel of eight gold-labeled lectins, we found that the cuticle exhibited slight labeling with all lectins used, indicating residues of N-acetyl-D: -glucosamine, N-acetyl-galactosamine, D: -galactose, D: -manose, and L: -fucose. Surface anionic sites were visualized by using cationized ferritin particles. Treatment with trypsin partially inhibited the reaction, whereas the treatment with chondroitinase ABC, a specific enzyme for glycosaminoglycans, completely abolished the labeling with cationic particles.
在本论文中，我们报告了使用几种细胞化学技术分析班氏吴策线虫感染性幼虫所获得的结果。使用咪唑四氧化锇溶液来观察不饱和脂肪酸。在幼虫表面和表皮上观察到一种形成连续结构的高对比度物质。在食管以及内分泌物质上观察到强烈反应。在采用蒂埃里技术处理的班氏吴策线虫第三期幼虫的角质层上未观察到含有邻二醇基团的碳水化合物。使用一组八种金标记凝集素，我们发现角质层对所有使用的凝集素都呈现轻微标记，表明存在N - 乙酰 - D - 葡萄糖胺、N - 乙酰 - 半乳糖胺、D - 半乳糖、D - 甘露糖和L - 岩藻糖残基。通过使用阳离子化铁蛋白颗粒观察表面阴离子位点。用胰蛋白酶处理可部分抑制反应，而用针对糖胺聚糖的特异性酶软骨素酶ABC处理则完全消除了阳离子颗粒的标记。
