Araujo A, Souto-Padron T, de Souza W
Laboratorio de Ultraestrutura Celular Hertha Meyer, Instituto de Biofisica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Brazil.
Int J Parasitol. 1995 May;25(5):569-77. doi: 10.1016/0020-7519(94)00167-m.
Because of the practical limitations of obtaining viable adult forms of the Wuchereria bancrofti, the major species responsible for human lymphatic filariasis, only few ultrastructural studies were carried out. Adult worms present the cuticle as the interface structure between host and parasite. Cuticle structure and the demonstration of the presence of basic proteins, lipids, small amounts of terminal carbohydrate residues, phospholipids and collagen in the cuticle was undertaken on thin sections of embedded parasites. Using immunocytochemical methods, antigenic epitopes similar to those found in the extra cellular matrix of vertebrates were localized on thin sections of the Lowicryl embedded adult filariae.
由于获取导致人类淋巴丝虫病的主要病原体——班氏吴策线虫的成年活体存在实际限制，因此仅开展了少量超微结构研究。成虫的角质层是宿主与寄生虫之间的界面结构。对包埋寄生虫的薄片进行了角质层结构研究，并证实角质层中存在碱性蛋白、脂质、少量末端碳水化合物残基、磷脂和胶原蛋白。使用免疫细胞化学方法，在低温包埋成年丝虫的薄片上定位了与脊椎动物细胞外基质中发现的抗原表位相似的抗原表位。
