Brayton Kelly A, Palmer Guy H, Brown Wendy C
Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99163-7040, USA.
Expert Rev Vaccines. 2006 Feb;5(1):95-101. doi: 10.1586/14760584.5.1.95.
Outer membrane protein preparations have been demonstrated to elicit protective immunity for Anaplasma marginale. Attempts to recreate this protective immunity using known surface proteins have been unsuccessful; therefore, novel outer membrane proteins have been searched for using a proteomic/genomic approach. Annotation of the whole genome sequence identified 62 outer membrane protein candidates based on sequence similarity to known surface proteins. In a proteomics approach for the identification of immunostimulatory outer membrane proteins, outer membrane preparations that were separated on 2D gels were used to immunize calves . Antisera from the calves were used to detect immunoreactive proteins, which were then selected and subjected to mass spectrometric analyses. These data were mapped back to the annotated A. marginale genome and have identified several new outer membrane proteins that are vaccine candidates.
已证明外膜蛋白制剂可引发针对边缘无形体的保护性免疫。使用已知表面蛋白重建这种保护性免疫的尝试均未成功；因此，已采用蛋白质组学/基因组学方法寻找新型外膜蛋白。对全基因组序列的注释基于与已知表面蛋白的序列相似性鉴定出62种外膜蛋白候选物。在用于鉴定免疫刺激性外膜蛋白的蛋白质组学方法中，在二维凝胶上分离的外膜制剂用于免疫小牛。来自小牛的抗血清用于检测免疫反应性蛋白，然后选择这些蛋白并进行质谱分析。这些数据被映射回注释的边缘无形体基因组，并已鉴定出几种作为疫苗候选物的新外膜蛋白。
