Ulucesme Mehmet Can, Ozubek Sezayi, Aktas Munir
Department of Parasitology, Faculty of Veterinary Medicine, University of Firat, Elazig 23119, Türkiye.
Life (Basel). 2023 Apr 28;13(5):1101. doi: 10.3390/life13051101.
is a tick-borne obligated intraerythrocytic bacterium that infects domestic sheep, goats, and wild ruminants. Recently, several studies have been carried out using and genes to identify the genetic diversity of . Instead of these genes, which are known to be highly stable among heterologous strains, , which is accepted as a stable molecular marker to classify strains, was used in genetic diversity studies. The genetic diversity of strains according to the gene has not been extensively reported. Therefore, the purpose of this study was to examine the genetic diversity of in goats specifically using analysis of the gene. Blood samples were taken from the vena jugularis to the EDTA tubes from 293 randomly selected goats (apparently healthy) in the Antalya and Mersin provinces of Mediterranean region of Türkiye. The gene of was amplified in all DNA samples through the use of PCR, using a specific set of primers named AoMsp1aF and AoMsp1aR. Among the amplified products, well-defined bands with different band sizes were subjected to sequence analysis. The obtained sequence data were converted into amino acid sequences using an online bioinformatics program and the tandem regions were examined. The gene of was amplified in 46.1% (135 out of 293) of the goats. Through tandem analysis, five distinct tandems (Ao8, Ao18, Tr15-16-17) were identified, and it was found that three of these tandems (Tr15-16-17) were previously unknown and were therefore defined as new tandems. The study also involved examination of ticks from goats. It was observed that the goats in the area were infested with several tick species, including (888/1091, 81.4%), (96/1091, 8.8%), (92/1091, 8.4%), (9/1091, 0.8%), and s.l. (6/1091, 0.5%). This study provides important data for understanding the genetic diversity and evolution of based on tandem repeats in the Msp1a protein.
是一种蜱传播的专性红细胞内细菌，可感染家养绵羊、山羊和野生反刍动物。最近，已经开展了几项使用 和 基因来鉴定 遗传多样性的研究。在异源菌株中已知高度稳定的这些基因之外，被认为是用于分类 菌株的稳定分子标记的 ，被用于 遗传多样性研究。根据 基因的 菌株遗传多样性尚未有广泛报道。因此，本研究的目的是专门使用 基因分析来检测山羊中 的遗传多样性。从土耳其地中海地区安塔利亚省和梅尔辛省随机挑选的293只（表面健康）山羊的颈静脉采集血样至乙二胺四乙酸（EDTA）管中。使用一组名为AoMsp1aF和AoMsp1aR的特异性引物，通过聚合酶链反应（PCR）在所有DNA样本中扩增 的 基因。在扩增产物中，对具有不同条带大小的清晰条带进行序列分析。使用在线生物信息学程序将获得的序列数据转换为氨基酸序列并检查串联区域。 在46.1%（293只中的135只）的山羊中扩增出 基因。通过串联分析，鉴定出五个不同的串联体（Ao8、Ao18、Tr15 - 16 - 17），并且发现其中三个串联体（Tr15 - 16 - 17）以前未知，因此被定义为新的串联体。该研究还涉及检查来自山羊的蜱。观察到该地区的山羊感染了几种蜱虫物种，包括 （888/1091，81.4%）、 （96/1091，8.8%）、 （92/1091，8.4%）、 （9/1091，0.8%）和 狭义种（6/1091，0.5%）。本研究为基于Msp1a蛋白串联重复理解 的遗传多样性和进化提供了重要数据。
