Iwamoto Tomotada, Saito Hajime
Department of Microbiology, Kobe Institute of Health, Kobe, Japan.
FEMS Microbiol Lett. 2006 Jan;254(1):129-33. doi: 10.1111/j.1574-6968.2005.00013.x.
One hundred and ninety-eight clinical isolates of Mycobacterium kansasii collected between 2003 and 2004 in Japan were genotyped by PCR and restriction enzyme analysis (PRA) and 16S-23S internal transcribed spacer (ITS) sequencing. The results demonstrated that clinical isolates of M. kansasii in Japan are almost exclusively of the type I PRA genotype, as is the case in other countries. Although the results of subtyping using the 16S-23S ITS sequence were generally consistent with subtyping using hsp65 PRA, four strains showed a discrepancy between the two methods. Sequence analysis of the hsp65, gyrB and 16S rRNA genes and the ITS sequence of the four strains suggests that they branched from type II and could be considered an ancestral strain of the type I strain. The newly recognized strains were designated as intermediate type I.
2003年至2004年期间在日本收集的198株堪萨斯分枝杆菌临床分离株，通过聚合酶链反应（PCR）和限制性内切酶分析（PRA）以及16S - 23S内部转录间隔区（ITS）测序进行基因分型。结果表明，日本的堪萨斯分枝杆菌临床分离株几乎均为I型PRA基因型，其他国家的情况也是如此。虽然使用16S - 23S ITS序列进行亚型分析的结果与使用hsp65 PRA进行亚型分析的结果总体一致，但有4株菌株在两种方法之间存在差异。对这4株菌株的hsp65、gyrB和16S rRNA基因以及ITS序列进行分析表明，它们从II型分支出来，可被视为I型菌株的祖先菌株。新识别出的菌株被指定为中间I型。
