Richter E, Niemann S, Rüsch-Gerdes S, Hoffner S
Forschungszentrum Borstel, National Reference Center for Mycobacteria, Borstel, Germany.
J Clin Microbiol. 1999 Apr;37(4):964-70. doi: 10.1128/JCM.37.4.964-970.1999.
The newly formulated Mycobacterium kansasii AccuProbe was evaluated, and the results obtained with the new version were compared to the results obtained with the old version of this test by using 116 M. kansasii strains, 1 Mycobacterium gastri strain, and 19 strains of several mycobacterial species. The sensitivity of this new formulation was 97.4% and the specificity was 100%. Still, three M. kansasii strains were missed by this probe. To evaluate the variability within the species, genetic analyses of the hsp65 gene, the spacer sequence between the 16S and 23S rRNA genes, and the 16S rRNA gene of several M. kansasii AccuProbe-positive strains as well as all AccuProbe-negative strains were performed. Genetic analyses of the one M. gastri strain from the comparative assay and of two further M. gastri strains were included because of the identity of the 16S rRNA gene in M. gastri to that in M. kansasii. The data confirmed the genetic heterogeneity of M. kansasii. Furthermore, a subspecies with an unpublished hsp65 restriction pattern and spacer sequence was described. The genetic data indicate that all M. kansasii strains missed by the AccuProbe test belong to one subspecies, the newly described subspecies VI, as determined by the hsp65 restriction pattern and the spacer sequence. Since the M. kansasii strains that are missed are rare and all M. gastri strains are correctly negative, the new formulated AccuProbe provides a useful tool for the identification of M. kansasii.
对新配制的堪萨斯分枝杆菌核酸探针进行了评估，并使用116株堪萨斯分枝杆菌菌株、1株胃分枝杆菌菌株和19株几种分枝杆菌属菌株，将该新版本检测获得的结果与旧版本检测获得的结果进行了比较。这种新制剂的敏感性为97.4%，特异性为100%。尽管如此，该探针仍遗漏了3株堪萨斯分枝杆菌菌株。为了评估该菌种内的变异性，对几株堪萨斯分枝杆菌核酸探针检测呈阳性的菌株以及所有核酸探针检测呈阴性的菌株的hsp65基因、16S和23S rRNA基因之间的间隔序列以及16S rRNA基因进行了遗传分析。由于胃分枝杆菌的16S rRNA基因与堪萨斯分枝杆菌的16S rRNA基因相同，因此纳入了比较试验中的1株胃分枝杆菌菌株以及另外2株胃分枝杆菌菌株的遗传分析。数据证实了堪萨斯分枝杆菌的遗传异质性。此外，还描述了一个具有未发表的hsp65限制性图谱和间隔序列的亚种。遗传数据表明，核酸探针检测遗漏的所有堪萨斯分枝杆菌菌株都属于一个亚种，即根据hsp65限制性图谱和间隔序列新描述的亚种VI。由于遗漏的堪萨斯分枝杆菌菌株很少见，且所有胃分枝杆菌菌株核酸探针检测均呈正确的阴性，因此新配制的核酸探针为堪萨斯分枝杆菌的鉴定提供了一个有用的工具。
