多重聚合酶链反应检测儿童大肠杆菌的毒力因子
Detection of virulence factors of Escherichia coli from children by multiplex polymerase chain reaction.
作者信息
Arisoy M, Aysev D, Ekim M, Ozel D, Köse S K, Ozsoy E D, Akar N
机构信息
Ankara University, Faculty of Health Education, Department of Basic Health Sciences, Ankara, Turkey.
出版信息
Int J Clin Pract. 2006 Feb;60(2):170-3. doi: 10.1111/j.1742-1241.2005.00668.x.
A total of 161 Escherichia coli (E. coli) strains isolated from children with urinary tract infection (UTI) were analysed for the genes encoding the virulence factors such as pyelonephritis (pap), s fimbriae (sfa), afimbrial adhesin I (afaI), haemolysin (hly), cytotoxic necrotising factor I (cnf I) and aerobactin (aer) by multiplex PCR. Ninety-four E. coli strains were found to carry at least one virulence factor. Therefore, 58.38% of total population was positive for one virulence gene at least. Percentage of genes within the total population for pap, sfa, afaI, hly, cnf I and aer was found as 22.98, 6.21, 9.94, 1.24, 9.94 and 39.75, respectively. Our analysis showed that sfa-pap (p < 0.001); pap-aer, afaI-aer and cnf I-pap (P < 0.05) and hly-sfa (p < 0.01) significantly co-occurred in their respective samples. In the light of these findings, we suggest an important role of pap causing UTI.
采用多重聚合酶链反应（PCR）对从患尿路感染（UTI）的儿童中分离出的161株大肠杆菌（E. coli）菌株进行分析，以检测编码诸如肾盂肾炎（pap）、S菌毛（sfa）、无纤毛黏附素I（afaI）、溶血素（hly）、细胞毒性坏死因子I（cnf I）和气杆菌素（aer）等毒力因子的基因。发现94株大肠杆菌菌株携带至少一种毒力因子。因此，总菌株数中有58.38%至少对一种毒力基因呈阳性。在总菌株数中，pap、sfa、afaI、hly、cnf I和aer基因的百分比分别为22.98%、6.21%、9.94%、1.24%、9.94%和39.75%。我们的分析表明，sfa与pap（p < 0.001）；pap与aer、afaI与aer以及cnf I与pap（P < 0.05）以及hly与sfa（p < 0.01）在各自样本中显著共现。根据这些发现，我们认为pap在引起UTI中起重要作用。
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