Wojnicz Dorota, Kucharska Alicja Z, Sokół-Łętowska Anna, Kicia Marta, Tichaczek-Goska Dorota
Department of Biology and Medical Parasitology, Wrocław Medical University, Mikulicza-Radeckiego 9, 50-367, Wrocław, Poland.
Urol Res. 2012 Dec;40(6):683-97. doi: 10.1007/s00240-012-0499-6. Epub 2012 Aug 23.
Medicinal plants are an important source for the therapeutic remedies of various diseases including urinary tract infections. This prompted us to perform research in this area. We decided to focus on medicinal plants species used in urinary tract infections prevention. The aim of our study was to determine the influence of Betula pendula, Equisetum arvense, Herniaria glabra, Galium odoratum, Urtica dioica, and Vaccinium vitis-idaea extracts on bacterial survival and virulence factors involved in tissue colonization and biofilm formation of the uropathogenic Escherichia coli rods. Qualitative and quantitative analysis of plant extracts were performed. Antimicrobial assay relied on the estimation of the colony forming unit number. Hydrophobicity of cells was established by salt aggregation test. Using motility agar, the ability of bacteria to move was examined. The erythrocyte hemagglutination test was used for fimbriae P screening. Curli expression was determined using YESCA agar supplemented with congo red. Quantification of biofilm formation was carried out using a microtiter plate assay and a spectrophotometric method. The results of the study indicate significant differences between investigated extracts in their antimicrobial activities. The extracts of H. glabra and V. vitis-idaea showed the highest growth-inhibitory effects (p < 0.05). Surface hydrophobicity of autoaggregating E. coli strain changed after exposure to all plant extracts, except V. vitis-idaea (p > 0.05). The B. pendula and U. dioica extracts significantly reduced the motility of the E. coli rods (p < 0.05). All the extracts exhibited the anti-biofilm activity.
药用植物是包括尿路感染在内的各种疾病治疗药物的重要来源。这促使我们在该领域开展研究。我们决定专注于用于预防尿路感染的药用植物物种。我们研究的目的是确定欧洲白桦、问荆、光萼墙草、香车叶草、异株荨麻和越橘提取物对参与尿路致病性大肠杆菌杆菌组织定植和生物膜形成的细菌存活及毒力因子的影响。对植物提取物进行了定性和定量分析。抗菌测定依赖于对菌落形成单位数量的估计。通过盐聚集试验确定细胞的疏水性。使用运动性琼脂检测细菌的运动能力。采用红细胞血凝试验筛查菌毛P。使用添加刚果红的YESCA琼脂测定卷曲菌毛的表达。使用微量滴定板测定法和分光光度法对生物膜形成进行定量。研究结果表明，所研究的提取物在抗菌活性方面存在显著差异。光萼墙草和越橘的提取物表现出最高的生长抑制作用（p < 0.05）。除越橘外，暴露于所有植物提取物后，自聚集大肠杆菌菌株的表面疏水性均发生变化（p > 0.05）。欧洲白桦和异株荨麻的提取物显著降低了大肠杆菌杆菌的运动能力（p < 0.05）。所有提取物均表现出抗生物膜活性。
