Black D N
J Gen Virol. 1975 Jan;26(1):109-19. doi: 10.1099/0022-1317-26-1-109.
Although the infection of BHK cells with foot-and-mouth disease virus did not cause a marked inhibition of cellular protein synthesis, the proteins synthesized gradually changed from host-specific to virus-specific. The synthesis of at least thirteen virus-induced proteins was demonstrated by polyacrylamide electrophoretic analysis of the infected cells. Only a small proportion of the virus-induced proteins was incorporated into the mature virus particles. The addition of amino acid analogues caused an accumulation of the larger mol. wt. proteins, suggesting that in infected cells, some cleavages occurred, giving rise to the smaller mol. wt. proteins. The operation of the precursor-cleavage mechanism was more clearly shown in pulse-chase experiments, in which it was found that the proteins were degraded from larger to smaller mol. wt.
虽然口蹄疫病毒感染BHK细胞并未显著抑制细胞蛋白质合成，但合成的蛋白质逐渐从宿主特异性转变为病毒特异性。通过对感染细胞进行聚丙烯酰胺电泳分析，证实至少有13种病毒诱导蛋白的合成。只有一小部分病毒诱导蛋白被整合到成熟病毒颗粒中。添加氨基酸类似物会导致较大分子量蛋白质的积累，这表明在感染细胞中发生了一些裂解，产生了较小分子量的蛋白质。前体裂解机制在脉冲追踪实验中表现得更为明显，实验发现蛋白质从较大分子量降解为较小分子量。
