Cleaves G R
J Gen Virol. 1985 Dec;66 ( Pt 12):2767-71. doi: 10.1099/0022-1317-66-12-2767.
Evidence is presented for the production in dengue type 2 virus (DEN-2)-infected BHK cells of large virus-specific proteins with molecular weights up to 250 000. These proteins were most prominent in lysates of cells which had been labelled with [35S]methionine for 7 to 15 min. During pulse-chase experiments, these high mol. wt. proteins appeared to be converted into smaller, more stable, proteins with mol. wt. between 10 000 and 98 000. Finally, inhibition of proteolysis prevented the chasing of label from the high mol. wt. proteins to the smaller viral proteins which normally accumulate in DEN-2-infected cells. These findings are consistent with the idea that processing of large polyprotein precursors plays an important role in the production of flavivirus proteins.
有证据表明，在登革2型病毒（DEN-2）感染的BHK细胞中产生了分子量高达250000的大型病毒特异性蛋白质。这些蛋白质在经[35S]甲硫氨酸标记7至15分钟的细胞裂解物中最为显著。在脉冲追踪实验中，这些高分子量蛋白质似乎转化为分子量在10000至98000之间的更小、更稳定的蛋白质。最后，蛋白水解的抑制阻止了标记从高分子量蛋白质转移到通常在DEN-2感染细胞中积累的较小病毒蛋白质上。这些发现与大型多蛋白前体的加工在黄病毒蛋白产生中起重要作用的观点一致。
