Department of Epidemiology, Bloomberg School of Public Health, 615 North Work Street, Johns Hopkins University, Baltimore, Maryland 21205, USA.
BMC Genet. 2005 Dec 30;6 Suppl 1(Suppl 1):S68. doi: 10.1186/1471-2156-6-S1-S68.
We used the FBAT (family-based association test) software to test for association between 300 individual single-nucleotide polymorphisms and P1 (a latent trait of Kofendred Personality Disorder) in 100 simulated replicates of the Aipotu population. Using the Genetic Analysis Workshop 14 dataset, we calculated the power of FBAT to detect linkage disequilibrium on chromosome 3 (D2). Also, we calculated the false-positive rate on chromosome 1, which contains a true locus (D1) but no linkage disequilibrium was simulated between the trait and all the surrounding single-nucleotide polymorphisms.
We were able to detect the associations between phenotype P1 and three adjacent markers B03T3056 (average p-value = 0.0002), B03T3057 (average p-value = 0.00072), and B03T3058 (average p-value = 0.0038) with power of 98%, 87%, 71% on chromosome 3, respectively. The overall false positive rate to detect association was 0.06 on chromosome 1.
The power to detect a significant association in 100 nuclear families affected with the latent trait of Kofendred Personality Disorder by using FBAT was reasonable (based on 100 replicates). In the future, we will compare the performance of FBAT with alternative approaches, such as using FBAT-generalized estimating equations methods to test for association in families affected with complex traits.
我们使用 FBAT（基于家庭的关联测试）软件在 100 个 Aipotu 人群的模拟重复中测试了 300 个个体单核苷酸多态性与 P1（Kofendred 人格障碍的潜在特征）之间的关联。使用遗传分析研讨会 14 数据集，我们计算了 FBAT 在检测染色体 3（D2）上连锁不平衡的功效。此外，我们计算了染色体 1 上的假阳性率，该染色体包含一个真实基因座（D1），但在性状和所有周围的单核苷酸多态性之间没有模拟出连锁不平衡。
我们能够检测到表型 P1 与三个相邻标记 B03T3056（平均 p 值=0.0002）、B03T3057（平均 p 值=0.00072）和 B03T3058（平均 p 值=0.0038）之间的关联，在染色体 3 上的功效分别为 98%、87%和 71%。检测关联的总体假阳性率在染色体 1 上为 0.06。
使用 FBAT 在受 Kofendred 人格障碍潜在特征影响的 100 个核家庭中检测到显著关联的功效是合理的（基于 100 个重复）。将来，我们将比较 FBAT 与替代方法（如使用 FBAT-广义估计方程方法在受复杂特征影响的家庭中检测关联）的性能。
