Sun Rhodes Lisa S, Merzdorf Christa S
Department of Cell Biology and Neuroscience, Montana State University, 513 Leon Johnson Hall, Bozeman, MT 59717, USA.
Gene Expr Patterns. 2006 Jun;6(5):539-45. doi: 10.1016/j.modgep.2005.10.006. Epub 2006 Jan 31.
Zic transcription factors regulate the expression of neural and neural crest-specific genes and are expressed in the cells of the dorsal neural tube and the premigratory neural crest. Here we characterize zic1 expression in the chick embryo during somite formation and neural crest migration. zic1 is expressed in the dorsomedial portion of epithelial somites and subsequently in the dorsomedial lip of the dermomyotome. Although zic1 is expressed in cells of the nascent myotome, it is absent from differentiated myotome cells that express myosin. As the dorsal root ganglia form, zic1 is expressed at high levels in the dorsal sclerotome and zic1 expression is more pronounced in the caudal regions of the somites. Double-label experiments showed that cells expressing zic1 are not labeled by the HNK-1 antibody specific for migratory neural crest cells. Thus, migrating neural crest cells do not express zic1.
锌指（Zic）转录因子调控神经及神经嵴特异性基因的表达，并在背侧神经管和迁移前神经嵴的细胞中表达。在此，我们描述了鸡胚中体节形成和神经嵴迁移过程中zic1的表达情况。zic1在上皮性体节的背内侧部分表达，随后在生皮节的背内侧缘表达。尽管zic1在新生肌节的细胞中表达，但在表达肌球蛋白的分化肌节细胞中不表达。随着背根神经节的形成，zic1在背侧体节中高水平表达，且zic1在体节的尾侧区域表达更明显。双重标记实验表明，表达zic1的细胞不会被针对迁移神经嵴细胞的HNK-1抗体标记。因此，迁移的神经嵴细胞不表达zic1。
