Filatova E A, Krapivinsky G B, Filatov G N, Lazareva A V, Fesenko E E
Laboratory of Biophysics of the Reception, USSR Academy of Sciences, Pushchino, Moscow Region.
Biochim Biophys Acta. 1991 May 7;1064(2):293-6. doi: 10.1016/0005-2736(91)90314-x.
Affinity-purified polyclonal anti-cGMP antibodies were obtained from rabbit serum after immunization by succinyl derivative of cGMP coupled to bovine serum albumin. These antibodies were used to raise antiidiotypic antibodies in rats. Putative antiidiotypic serum inhibited the binding of [3H]cGMP to affinity-purified anti-cGMP antibodies. The influence of immunoglobulins isolated from antiidiotypic serum on the ion conductance of rod outer segment plasma membrane fragments from frog retina was studied in patch-clamp experiments. These immunoglobulins increased the conductance of ion channels acting like a natural agonist (cGMP). Preimmune immunoglobulins did not act. The data obtained suggest that antiidiotypic antibodies interact with regulatory cGMP-binding sites of the plasma membrane channels.
通过用与牛血清白蛋白偶联的cGMP琥珀酰衍生物免疫后，从兔血清中获得亲和纯化的多克隆抗cGMP抗体。这些抗体用于在大鼠中产生抗独特型抗体。推测的抗独特型血清抑制[3H]cGMP与亲和纯化的抗cGMP抗体的结合。在膜片钳实验中研究了从抗独特型血清中分离的免疫球蛋白对青蛙视网膜杆状外段质膜片段离子电导的影响。这些免疫球蛋白增加了类似天然激动剂（cGMP）作用的离子通道的电导。免疫前的免疫球蛋白没有作用。获得的数据表明抗独特型抗体与质膜通道的调节性cGMP结合位点相互作用。
