Neves M J, Jorge J A, François J M, Terenzi H F
Departamento de Biologia, Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto, Universidade de São Paulo, Brazil.
FEBS Lett. 1991 May 20;283(1):19-22. doi: 10.1016/0014-5793(91)80544-d.
Neurospora crassa conidiospore germlings exposed to a heat shock (30-45 C) rapidly accumulated trehalose and degraded glycogen, even in the presence of cycloheximide. This phenomenon was also rapidly reversible upon return of the cells at 30 degrees C. Trehalose accumulation at 45 degrees C demanded an exogenous source of carbon and either glucose or glycerol fulfilled such requirement. Experiments with the cyclic AMP-deficient cr-1 mutant suggested that the effects of temperature shifts on trehalose level were independent of cAMP metabolism. Cells exposed at 45 degrees C under conditions permissive for trehalose accumulation (i.e. in the presence of an assimilable carbon source) also acquired thermotolerance.
暴露于热激（30 - 45℃）的粗糙脉孢菌分生孢子萌发管即使在存在环己酰亚胺的情况下也会迅速积累海藻糖并降解糖原。当细胞回到30℃时，这种现象也能迅速逆转。45℃时海藻糖的积累需要外源碳源，葡萄糖或甘油均可满足这一需求。对缺乏环腺苷酸的cr - 1突变体进行的实验表明，温度变化对海藻糖水平的影响与环腺苷酸代谢无关。在允许海藻糖积累的条件下（即在存在可同化碳源的情况下）于45℃暴露的细胞也获得了耐热性。
