De Virgilio C, Simmen U, Hottiger T, Boller T, Wiemken A
Department of Botany, University of Basel, Switzerland.
FEBS Lett. 1990 Oct 29;273(1-2):107-10. doi: 10.1016/0014-5793(90)81062-s.
Exponentially growing cells of the fission yeast, Schizosaccharomyces pombe, contained virtually no trehalose at 27 degrees C but rapidly accumulated large quantities during heat shock at 40 degrees C. Activities of trehalose-6-phosphate synthase and trehalase also increased upon heat shock. Thermotolerance of the cells, measured as survival at 52 degrees C, increased in parallel to trehalose accumulation and decreased in parallel to the trehalose levels when cells were shifted back to 27 degrees C. Trehalose levels, activities of enzymes of trehalose metabolism and thermotolerance strongly increased upon heat shock even in the presence of cycloheximide, indicating that none of these effects requires protein synthesis. The data support the hypothesis that trehalose acts as a thermoprotectant in Schizosaccharomyces pombe.
裂殖酵母粟酒裂殖酵母指数生长的细胞在27℃时几乎不含海藻糖，但在40℃热激期间迅速积累大量海藻糖。海藻糖-6-磷酸合酶和海藻糖酶的活性在热激时也增加。以52℃存活率衡量的细胞耐热性与海藻糖积累平行增加，当细胞转回27℃时，与海藻糖水平平行降低。即使存在环己酰亚胺，热激时海藻糖水平、海藻糖代谢酶活性和耐热性也会强烈增加，表明这些效应均不需要蛋白质合成。这些数据支持了海藻糖在粟酒裂殖酵母中作为热保护剂的假说。
