Bard Frederic, Casano Laetitia, Mallabiabarrena Arrate, Wallace Erin, Saito Kota, Kitayama Hitoshi, Guizzunti Gianni, Hu Yue, Wendler Franz, Dasgupta Ramanuj, Perrimon Norbert, Malhotra Vivek
Cell and Developmental Biology Department, University of California San Diego, La Jolla, California 92093-0634, USA.
Nature. 2006 Feb 2;439(7076):604-7. doi: 10.1038/nature04377.
Yeast genetics and in vitro biochemical analysis have identified numerous genes involved in protein secretion. As compared with yeast, however, the metazoan secretory pathway is more complex and many mechanisms that regulate organization of the Golgi apparatus remain poorly characterized. We performed a genome-wide RNA-mediated interference screen in a Drosophila cell line to identify genes required for constitutive protein secretion. We then classified the genes on the basis of the effect of their depletion on organization of the Golgi membranes. Here we show that depletion of class A genes redistributes Golgi membranes into the endoplasmic reticulum, depletion of class B genes leads to Golgi fragmentation, depletion of class C genes leads to aggregation of Golgi membranes, and depletion of class D genes causes no obvious change. Of the 20 new gene products characterized so far, several localize to the Golgi membranes and the endoplasmic reticulum.
酵母遗传学和体外生化分析已鉴定出许多参与蛋白质分泌的基因。然而，与酵母相比，后生动物的分泌途径更为复杂，许多调节高尔基体组织的机制仍未得到充分表征。我们在果蝇细胞系中进行了全基因组RNA介导的干扰筛选，以鉴定组成型蛋白质分泌所需的基因。然后，我们根据基因缺失对高尔基体膜组织的影响对这些基因进行分类。在这里，我们表明，A类基因的缺失会使高尔基体膜重新分布到内质网中，B类基因的缺失会导致高尔基体碎片化，C类基因的缺失会导致高尔基体膜聚集，而D类基因的缺失则不会引起明显变化。在目前已表征的20种新基因产物中，有几种定位于高尔基体膜和内质网。
