[痘苗病毒基因组的分子生物学研究。II. 编码36K和12K蛋白的痘苗病毒基因的定位及核苷酸序列]
[Molecular-biological study of vaccinia virus genome. II. Localization and nucleotide sequence of vaccinia virus genes coding for proteins 36K and 12K].
作者信息
Riazankina O I, Shchelkunov S N, Muravlev A I, Netesova N A, Mikriukov N N, Gutorov V V, Nikulin A E, Kulichkov V A, Malygin E G
出版信息
Mol Biol (Mosk). 1990 Jul-Aug;24(4):968-76.
Genes encoding virus-specific late proteins with molecular mass 36 kDa and 12 kDa were mapped in HindIII-P DNA fragment of vaccinia virus strain L-IVP by hybrid selection of RNA to cloned DNA fragments followed by in vitro translation. RNA origin site of the 36K protein was detected in HindIII-J fragment. Nucleotide sequences of these genes were determined. Amino acid sequences of the 36K and 12K polypeptides were compared with the protein bank PIR.
通过将RNA与克隆的DNA片段进行杂交选择，随后进行体外翻译，在痘苗病毒L-IVP株的HindIII-P DNA片段中定位了编码分子量为36 kDa和12 kDa的病毒特异性晚期蛋白的基因。在HindIII-J片段中检测到36K蛋白的RNA起始位点。测定了这些基因的核苷酸序列。将36K和12K多肽的氨基酸序列与蛋白质库PIR进行了比较。
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