Plant Breeding Institute, Maris Lane, Trumpington, Cambridge CB2 2LQ, UK.
EMBO J. 1985 Aug;4(8):1921-6. doi: 10.1002/j.1460-2075.1985.tb03871.x.
The 35S transcript promoter of cauliflower mosaic virus directs the synthesis of an abundant RNA in infected leaf tissue that is both a template for reverse transcription during virus replication and also a polycistronic mRNA. The 35S promoter was isolated and linked to a cDNA of the 3' end of tobacco mosaic virus that encodes the coat protein of this single-stranded RNA virus. After transformation of this gene construction into tobacco plants using a disarmed Agrobacterium binary vector, viral coat protein synthesis was detected in transformed leaf tissue. A chimaeric 35S coat protein RNA was detected in transformed callus.
花椰菜花叶病毒的 35S 转录启动子指导受感染叶片组织中大量 RNA 的合成，该 RNA 既是病毒复制过程中逆转录的模板，也是多顺反子 mRNA。35S 启动子被分离出来，并与编码这种单链 RNA 病毒外壳蛋白的烟草花叶病毒 3' 端 cDNA 连接。使用一种无毒性的农杆菌二元载体将这个基因构建转化到烟草植物中后，在转化的叶片组织中检测到了病毒外壳蛋白的合成。在转化的愈伤组织中检测到了嵌合的 35S 外壳蛋白 RNA。
