Institute de Biologie Moléculaire des Plantes du CNRS and Université Louis Pasteur, Laboratoire de Virologie, 12 rue du Général Zimmer, F-67084, Strasbourg Cedex, France.
Plant Cell Rep. 1994 Mar;13(6):357-60. doi: 10.1007/BF00232637.
Grapevine fanleaf nepovirus (GFLV) is responsible for the economically significant "court-noué" disease in vineyards. Its genome is made up of two single-stranded RNA molecules (RNA1 and RNA2) which direct the synthesis of polyproteins P1 and P2 respectively. A chimeric coat protein gene derived from the C-terminal part of P2 was constructed and subsequently introduced into a binary transformation vector. Transgenic Nicotiana benthamiana plants expressing the coat protein under the control of the CaMV 35S promoter were engineered by Agrobacterium tumefaciens-mediated transformation. Protection against infection with virions or viral RNA was tested in coat protein-expressing plants. A significant delay of systemic invasion was observed in transgenic plants inoculated with virus compared to control plants. This effect was also observed when plants were inoculated with viral RNA. No coat protein-mediated cross-protection was observed when transgenic plants were infected with arabis mosaic virus (ArMV), a closely related nepovirus also responsible for a "court-noué" disease.
葡萄扇叶病毒（GFLV）是造成葡萄园经济上重要的“ court-noué”病害的原因。其基因组由两条单链 RNA 分子（RNA1 和 RNA2）组成，分别指导多蛋白 P1 和 P2 的合成。构建了源自 P2 末端部分的嵌合外壳蛋白基因，并随后将其引入二元转化载体。通过根癌农杆菌介导的转化工程，在 CaMV 35S 启动子的控制下表达外壳蛋白的转化拟南芥 Nicotiana benthamiana 植物。在表达外壳蛋白的植物中测试了对病毒粒子或病毒 RNA 感染的保护。与对照植物相比，用病毒接种的转基因植物中观察到系统侵染的明显延迟。当用病毒 RNA 接种植物时，也观察到这种效果。当用与葡萄扇叶病毒（GFLV）密切相关的芜菁黄花叶病毒（ArMV）感染转基因植物时，未观察到外壳蛋白介导的交叉保护。
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