Department of Molecular Biology, Agricultural University, De Dreijen 11, 6703 BC, Wageningen, The Netherlands.
Plant Mol Biol. 1986 May;7(3):171-6. doi: 10.1007/BF00021328.
Plant cells are sensitive to the antibiotic bleomycin, a DNA damaging glycopeptide. A bleomycin resistance determinant, located on transposon Tn5 and functional in bacteria, has been cloned in a plant expression vector and introduced into Nicotiana plumbaginifolia using Agrobacterium tumefaciens. The expression of this determinant in plant cells confers resistance to bleomycin and allows selection of transformed plant cells.
植物细胞对博莱霉素这种破坏 DNA 的糖肽类抗生素敏感。博莱霉素抗性决定簇位于转座子 Tn5 上，在细菌中具有功能，现已被克隆到植物表达载体中，并通过根癌农杆菌转入烟草(Nicotiana plumbaginifolia)。该决定簇在植物细胞中的表达赋予了细胞对博莱霉素的抗性，同时也允许转化植物细胞的选择。
