Institut für Genetik, Universität Düsseldorf, Universitätsstr. 1, D-4000 Düsseldorf 1, FRG.
EMBO J. 1986 Dec 20;5(13):3579-82. doi: 10.1002/j.1460-2075.1986.tb04685.x.
The accumulation of male-specific transcripts in various genotypes of Drosophila melanogaster was analysed by Northern blot hybridization. The genotypes were either hetero- or homozygous for a mutation in one of the genes for somatic sex determination. The aim was to study the role of these genes in regulating male-specific transcription in soma or germ line. All intersexual phenotypes and pseudomales irrespective of their genotypic sex accumulate the male-specific somatic RNA demonstrating that it is regulated by these genes. In contrast to this, the transcript from the male germ line could only be detected in those mutant genotypes that have the male sex chromosome constitution of X/Y, although its synthesis is not dependent on the presence of a Y chromosome. It is, therefore, not under the control of the sex determining loci but directly regulated by the primary signal for sex determination, namely the ratio of X chromosomes to autosomes.
通过 Northern 印迹杂交分析了不同果蝇黑色素体基因型中雄性特异性转录本的积累。这些基因型要么是某个体节性别决定基因发生突变的杂合子，要么是纯合子。目的是研究这些基因在调节体细胞或生殖系中雄性特异性转录中的作用。所有雌雄同体表型和拟雄体，无论其基因型性别如何，都积累了雄性特异性的体细胞 RNA，这表明它受到这些基因的调控。与此相反，来自雄性生殖系的转录本只能在那些具有 X/Y 性染色体组成的突变基因型中检测到，尽管其合成不依赖于 Y 染色体的存在。因此，它不受性别决定基因座的控制，而是直接受性别决定的主要信号（即 X 染色体与常染色体的比例）调控。
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