Kuhn R, Schäfer U, Schäfer M
Institut für Genetik, Universität Düsseldorf, FRG.
EMBO J. 1988 Feb;7(2):447-54. doi: 10.1002/j.1460-2075.1988.tb02832.x.
In Drosophila spermatogenesis transcription occurs only premeiotically while translation can be detected also in postmeiotic spermatids. To analyse the underlying processes mst(3)gl-9, a gene specifically expressed in the male germ cells of Drosophila melanogaster, was studied. The putative protein encoded by mst(3)gl-9 is mostly composed of repetitive Cys-Gly-Pro motifs. The transcriptional and translational control of expression of mst(3)gl-9 has been investigated by P-mediated transformation. Only 102 bp of 5' upstream sequences and the first 201 bp of the gene are sufficient to maintain the gene specific characteristics of expression, namely premeiotic transcription and postmeiotic translation separated by 3 days of development.
在果蝇精子发生过程中，转录仅发生在减数分裂前，而在减数分裂后的精子细胞中也能检测到翻译。为了分析潜在的过程，研究了mst(3)gl-9，这是一个在黑腹果蝇雄性生殖细胞中特异性表达的基因。mst(3)gl-9编码的推定蛋白质主要由重复的半胱氨酸-甘氨酸-脯氨酸基序组成。通过P介导的转化研究了mst(3)gl-9表达的转录和翻译控制。仅5'上游序列的102 bp和该基因的前201 bp就足以维持基因表达的特异性特征，即在发育3天后，减数分裂前转录和减数分裂后翻译分开。
