Department of Botany, University of Edinburgh, The King's Buildings, Mayfield Road, Edinburgh EH9 3JH, UK.
EMBO J. 1987 Apr;6(4):865-9. doi: 10.1002/j.1460-2075.1987.tb04832.x.
Four genomic arrangements of the maize mitochondrial atpA gene (encoding the alpha subunit of the F(1) ATPase), have been characterized. Most N (fertile) and S (male-sterile) cytoplasms contain two atpA arrangements of equal abundance. Prolonged exposure of blots of maize mitochondrial DNA probed with atpA-specific sequences show that cytoplasms previously reported to lack one of the atpA arrangements do contain the second arrangement but at low levels. Similarly, restriction fragments containing the atpA gene previously thought unique to male-sterile S and T cytoplasms are present in low abundance in fertile cytoplasms. These observations suggest that fertile and male-sterile cytoplasms of maize may be more closely related than previously thought, and suggest possible mechanisms to explain the observed mitochondrial genome diversity.
已鉴定出玉米线粒体 atpA 基因（编码 F（1）ATP 酶的α亚基）的四个基因组排列。大多数 N（可育）和 S（雄性不育）细胞质中含有两种丰度相等的 atpA 排列。用 atpA 特异性序列探测玉米线粒体 DNA 的印迹的长时间暴露表明，先前报道缺乏 atpA 排列之一的细胞质确实含有第二种排列，但水平较低。同样，先前认为仅存在于雄性不育 S 和 T 细胞质中的含有 atpA 基因的限制片段在可育细胞质中也以低丰度存在。这些观察结果表明，玉米的可育和雄性不育细胞质可能比以前认为的更为密切相关，并提出了可能的机制来解释观察到的线粒体基因组多样性。
