Soybean Research Institute, National Center for Soybean Improvement, Key Laboratory of Biology and Genetic Improvement of Soybean (General, Ministry of Agriculture), State Key Laboratory of Crop Genetics and Germplasm Enhancement, Jiangsu Collaborative Innovation Center for Modern Crop Production, College of Agriculture, Nanjing Agricultural University, Nanjing, 210095, China.
Soybean Genomics and Improvement Laboratory, Beltsville Agricultural Research Center, USDA-ARS, Beltsville, MD, 20705, USA.
Funct Integr Genomics. 2021 Jan;21(1):43-57. doi: 10.1007/s10142-020-00760-x. Epub 2021 Jan 6.
In soybean, only one mitochondrial genome of cultispecies has been completely obtained. To explore the effect of mitochondrial genome on soybean cytoplasmic male sterility (CMS), two CMS lines and three maintainer lines were used for sequencing. Comparative analysis showed that mitochondrial genome of the CMS line was more compact than that of its maintainer line, but genes were highly conserved. Conserved and unique sequence coexisted in the genomes. Mitochondrial genomes contained different sequence lengths and copy numbers of repeats between CMS line and maintainer line. Large and short repeats mediated intramolecular and intermolecular recombination in mitochondria. Unique sequences and genes were also involved in recombination process and constituted a complex network. orf178 and orf261 were identified as CMS-associated candidate genes. They had sequence characteristics of reported CMS genes in other crops and could be transcribed in CMS lines but not in maintainer lines. This report reveals mitochondrial genome of soybean CMS lines and compares complete mitochondrial sequence between CMS lines and their maintainer lines. The information will be helpful in further understanding the characteristics of soybean mitochondrial genome and the mechanism underlying CMS.
在大豆中，仅获得了一个栽培种的完整线粒体基因组。为了探讨线粒体基因组对大豆细胞质雄性不育（CMS）的影响，对两个 CMS 系和三个保持系进行了测序。比较分析表明，CMS 系的线粒体基因组比其保持系更为紧凑，但基因高度保守。基因组中既有保守序列，也有独特序列。CMS 系和保持系的线粒体基因组中重复序列的长度和拷贝数不同。大和小的重复序列介导了线粒体的分子内和分子间重组。独特序列和基因也参与了重组过程，构成了一个复杂的网络。orf178 和 orf261 被鉴定为与 CMS 相关的候选基因。它们具有其他作物报道的 CMS 基因的序列特征，可在 CMS 系中转录，但在保持系中不能转录。本报告揭示了大豆 CMS 系的线粒体基因组，并比较了 CMS 系与其保持系之间完整的线粒体序列。这些信息将有助于进一步了解大豆线粒体基因组的特征和 CMS 的机制。
